ArticleNature communications2022
Spatially resolved proteomics via tissue expansion.
Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 58 citations in OpenAlex.
- Multiomics analyses revealed the roles of the epithelial-mesenchymal transition and novel early candidate diagnostic biomarkers in neonatal necrotising enterocolitis.Clinical and translational medicine · 2026Article
- MALDI-MSI-Guided Laser Capture Microdissection Coupled with MS for Integrated Spatial Multi-Omics in Mouse Brain.Life (Basel, Switzerland) · 2026Article
- DeSpaST: deconvoluting spatial transcriptomics signals to cell-level resolution using histology images.Briefings in bioinformatics · 2026Article
- Multi-Tiered µDicer Enables Protein-Preserving Microdissection at 10 µm Resolution.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- SPOTTER: Automated Tissue-Barcoding Platform for Spatial Proteomics and Phosphoproteomics.bioRxiv : the preprint server for biology · 2026Article
- Protein S-Nitrosylation in Heart Failure: A Compartment-Resolved Review of Mechanisms, Evidence Boundaries, and Translational Perspectives.Antioxidants (Basel, Switzerland) · 2026Review
- Filter-aided expansion proteomics for the spatial analysis of single cells and organelles in FFPE tissue samples.Nature protocols · 2026Review
- Single-Cell Protein Assays in Context: From 2D to 3D and In Situ Analysis.Annual review of analytical chemistry (Palo Alto, Calif.) · 2026Review
- Neoadjuvant mFOLFOX6 plus camrelizumab and apatinib in colon cancer and biomarker research via spatiotemporal-omics profiling.NPJ precision oncology · 2026Article
- Review
- Spatial Proteomics Using S4P.Bio-protocol · 2026Article
- The good, the bad, and the ugly: opportunities, challenges, and pitfalls in spatial proteomics modeling.Briefings in bioinformatics · 2026Review
- Expansion omics: from expansion microscopy to spatial omics.Molecular systems biology · 2026Review
- Advances in the pathophysiological study of brain development: application of cerebral organoid combined with Spatial omics technology.Stem cell research & therapy · 2026Review
- Article
- Spatial proteomics: technical evolution and applications in cancer research.Frontiers in cell and developmental biology · 2026Review
- Hyperactivity of subicular parvalbumin interneurons drives early amyloid pathology and cognitive deficits in Alzheimer's disease.Molecular psychiatry · 2025Article
- PIWI proteins tether the piRNA biogenesis machinery to mitochondria during mammalian spermatogenesis.The EMBO journal · 2025Article
- The Safety and Performance of a Novel Extracorporeal Membrane Oxygenation Device in a Long-Term Ovine Model.Advances in respiratory medicine · 2025Article
- Spatially resolved C1QCCell discovery · 2025Article
Corrections and comments
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Authors and funding
15 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Spatially resolved proteomics is an emerging approach for mapping proteome heterogeneity of biological samples, however, it remains technically challenging due to the complexity of the tissue microsampling techniques and mass spectrometry analysis of nanoscale specimen volumes. Here, we describe a spatially resolved proteomics method based on the combination of tissue expansion with mass spectrometry-based proteomics, which we call Expansion Proteomics (ProteomEx). ProteomEx enables quantitative profiling of the spatial variability of the proteome in mammalian tissues at ~160 µm lateral resolution, equivalent to the tissue volume of 0.61 nL, using manual microsampling without the need for custom or special equipment. We validated and demonstrated the utility of ProteomEx for streamlined large-scale proteomics profiling of biological tissues including brain, liver, and breast cancer. We further applied ProteomEx for identifying proteins associated with Alzheimer's disease in a mouse model by comparative proteomic analysis of brain subregions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.