Evidence map›Paper›PMID 36446972›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

A Mass Spectrometry-Based Strategy for Mapping Modification Sites for the Ubiquitin-Like Modifier NEDD8.

Caio A B Oliveira, Ekaterina Isaakova, Petra Beli, Dimitris P Xirodimas

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.5field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 2 countries.

Caio A B Oliveira *Institute of Molecular Biology (IMB), Mainz, Germany.
Ekaterina Isaakova *Institute of Molecular Biology (IMB), Mainz, Germany.
Petra BeliInstitute of Molecular Biology (IMB), Mainz, Germany. p.beli@imb-mainz.de.
Dimitris P XirodimasCRBM, University of Montpellier, CNRS, Montpellier, France. dimitris.xirodimas@crbm.cnrs.fr.
Institute of Molecular Biology · DEJohannes Gutenberg University Mainz · DEUniversité de Montpellier · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The identification of modification sites for ubiquitin and ubiquitin-like modifiers is an essential step in the elucidation of controlled processes. The ubiquitin-like modifier NEDD8 is an important regulator of plethora of biological processes both under homeostatic and proteotoxic stress conditions. Here, we describe a detailed protocol for proteome-wide identification of NEDDylation sites. The approach is based on the use of cell lines stably expressing the NEDD8

Indexed as

FabaceaeUbiquitinChromatography, LiquidGlycineProteomeTandem Mass SpectrometryGlycineProteomeUbiquitinDi-glycine remnantHigh-throughput analysisMass spectrometryNEDD8Ubiquitin-like modifiers

Identifiers

PMID36446972
OpenAlexW4310345273

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.