Evidence map›Paper›PMID 36446966›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Total Linear Chemical Synthesis of LC3A and LC3B.

Yara Huppelschoten, Jens Buchardt, Thomas Eiland Nielsen, Gerbrand J van der Heden van Noort

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

Yara HuppelschotenOncode Institute and Department of Cell and Chemical Biology, Leiden University Medical Centre, Leiden, The Netherlands.
Jens BuchardtGlobal Research Technologies, Novo Nordisk, Novo Nordisk Park, Måløv, Denmark.
Thomas Eiland NielsenGlobal Research Technologies, Novo Nordisk, Novo Nordisk Park, Måløv, Denmark.
Gerbrand J van der Heden van NoortOncode Institute and Department of Cell and Chemical Biology, Leiden University Medical Centre, Leiden, The Netherlands. gvanderheden@lumc.nl.
Novo Nordisk (Denmark) · DKOncode Institute · NL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Solid-phase peptide synthesis (SPPS) enables the synthesis of chemically modified peptides and proteins. Chemically synthesized ubiquitin(-like) proteins containing a fluorescent tag or reactive warhead have proven to be important tools in elucidating biological processes. Here, we describe the first fully synthetic method for the linear synthesis of two LC3 ubiquitin-like proteins using disaggregating building blocks and heated synthesis. Both LC3A and LC3B were synthesized and equipped with a fluorescent rhodamine tag, followed by folding of the proteins and liquid chromatography-mass spectrometry and SDS-PAGE analysis to prove that the quality of the synthetic material is comparable to expressed material.

Indexed as

Coloring AgentsHot TemperatureChromatography, LiquidElectrophoresis, Polyacrylamide GelMass SpectrometryUbiquitinsColoring AgentsUbiquitinsChemically modified proteinsChemical protein synthesisLC3Solid phase peptide synthesisUbiquitin-like proteins

Identifiers

PMID36446966
OpenAlexW4310702221

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.