ArticleScientific reports2022
Sensitive and rapid detection of Babesia species in dogs by recombinase polymerase amplification with lateral flow dipstick (RPA-LFD).
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 27 citations in OpenAlex.
- A recombinase polymerase amplification lateral flow assay (RPA-LF) for detection of Babesia divergens-like parasites.Parasites & vectors · 2026Article
- Development and evaluation of a lateral flow assay for detecting Anaplasma marginale infection in cattle.Veterinary research communications · 2026Article
- Development and field evaluation of a rapid detection method for Babesia microti using fluorescent recombinase polymerase amplification(RPA) technology.Scientific reports · 2026Article
- A novel rapid detection approach based on CRISPR-Cas13a for Dermatophagoides pteronyssinus and Dermatophagoides farinae (Acariformes: Pyroglyphidae).Journal of insect science (Online) · 2026Article
- A One-Pot RPA-CRISPR/Cas12a Assay for Rapid Genus-Level Detection of Babesia spp. in Ticks and Livestock Blood Samples.Transboundary and emerging diseases · 2026Article
- A comprehensive review of lateral flow assays in rapid livestock and poultry disease diagnostics.Journal of advanced veterinary and animal research · 2026Article
- CRISPR-Cas12a-based rapid detection of Babesia gibsoni and Ehrlichia canis in dogs using fluorometer platform.Scientific reports · 2025Article
- Comparative molecular detection and phylogenetic analysis ofVeterinary world · 2025Article
- Research progress on diagnostic techniques for differentFrontiers in cellular and infection microbiology · 2025Review
- Development of a rapid recombinase polymerase amplification-lateral flow dipstick assay for sensitive detection of duck adenovirus type-3.Frontiers in microbiology · 2025Article
- Rapid and Accurate Detection ofInsects · 2024Article
- Rapid and Ultrasensitive Detection ofMolecules (Basel, Switzerland) · 2024Article
- Recombinase-aid amplification combined with lateral flow detection assay for sex identification of the great white pelican (Pelecanus onocrotalus).Scientific reports · 2024Article
- Real-Time Monitoring on the Chinese Giant Salamander Using RPA-LFD.International journal of molecular sciences · 2024Article
- Recombinase polymerase amplification combined with lateral flow dipstick assay for rapid visual detection ofHeliyon · 2024Article
- Recombinase Polymerase Amplification Combined with Lateral Flow Dipstick Assay for the Rapid and Sensitive Detection ofInternational journal of molecular sciences · 2024Article
- A novel detection method based on MIRA-CRISPR/Cas13a-LFD targeting the repeated DNA sequence of Trichomonas vaginalis.Parasites & vectors · 2024Article
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Canine babesiosis is a tick-borne disease caused by Babesia spp., which infects and destroys healthy erythrocytes, leading to mortality and morbidity in dogs. The diagnosis of babesiosis is tedious and time-consuming, especially in latent and chronic infections. Here, a recombinase polymerase amplification combined with a lateral flow dipstick (RPA-LFD) assay was developed for rapid and accurate detection of Babesia spp. in canine blood specimens based on the 18S rRNA region. The RPA-LFD assay using rpaBab264 gave specificity to Babesia spp. in dogs (B. vogeli and B. gibsoni) without cross-amplification to other parasites (apicomplexans and non-apicomplexans), with detection limit of at least 22.5 copies/μl (0.1 fg/µl) at 40 °C for at least 10 min. The whole process of DNA amplification by RPA and readout by LFD did not exceed 30 min. To determine the performance of the RPA-LFD assay, a total of 30 clinical samples was examined and compared with conventional PCR (cPCR) and multiplex HRM (mHRM). Eight dogs (26.67%) were detected as positive by RPA-LFD, while seven and six were found positive by cPCR and mHRM, respectively. RPA-LFD and cPCR showed high agreement with Babesia spp. detection with kappa > 0.9. We confirmed that the dogs were infected by B. vogeli from sequences of positive PCR results. Our findings suggested that RPA-LFD using the rpaBab264 assay offered a rapid, accurate, cost-effective and simple method for Babesia spp. detection that is feasibly applicable to be rapid kit at a pet hospital or point-of-care testing.
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