Evidence map›Paper›PMID 36445596›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Functional Metagenomics to Study Antibiotic Resistance.

Bejan Mahmud, Manish Boolchandani, Sanket Patel, Gautam Dantas

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Antibiotic perturbations to the gut microbiome.Nature reviews. Microbiology · 2023
    Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Bejan MahmudThe Edison Family Center for Genome Sciences & Systems Biology, Washington University School of Medicine, St. Louis, MO, USA.
Manish BoolchandaniThe Edison Family Center for Genome Sciences & Systems Biology, Washington University School of Medicine, St. Louis, MO, USA.
Sanket PatelThe Edison Family Center for Genome Sciences & Systems Biology, Washington University School of Medicine, St. Louis, MO, USA.
Gautam DantasThe Edison Family Center for Genome Sciences & Systems Biology, Washington University School of Medicine, St. Louis, MO, USA. dantas@wustl.edu.

Funding

METAGENOMIC ENGINEERING OF PROBIOTIC BACTERIA TO IMPROVE INTESTINAL COLONIZATIONDP2DK098089 · NIDDK · WASHINGTON UNIVERSITY · PI DANTAS, GAUTAM · 2012 to 2012
$2.1M
ESTABLISHMENT AND DYNAMICS OF ANTIBIOTIC RESISTANCE RESERVOIRS IN DEVELOPING INTER01GM099538 · NIGMS · WASHINGTON UNIVERSITY · PI DANTAS, GAUTAM · 2013 to 2016
$1.2M
NIDDK NIH HHS DP2 DK098089NIGMS NIH HHS R01 GM099538
6 · The paper itself

Abstract

The construction and screening of metagenomic expression libraries have a great potential to identify novel genes with desired functions. Here, we describe metagenomic library preparation from fecal DNA, screening of libraries for antibiotic resistance genes (ARGs), massively parallel DNA sequencing of the enriched DNA fragments, and a computational pipeline for high-throughput assembly and annotation of functionally selected DNA.

Indexed as

MetagenomeMetagenomicsDrug Resistance, MicrobialGene LibrarySequence Analysis, DNAAntibiotic resistance genesFunctional metagenomicsFunctional selectionsHigh-throughput assemblyMassively parallel DNA sequencingPARFuMSProfile HMM-based annotationResfamsResistome

Identifiers

PMID36445596

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.