Evidence map›Paper›PMID 36445593›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Sample Preparation for Mass Spectrometry-Based Absolute Quantification of Bacterial Proteins in Antibiotic Stress Research.

Sandra Maaß, Minia Antelo-Varela, Florian Bonn, Dörte Becher

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 63% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

Sandra MaaßDepartment of Microbial Proteomics, University of Greifswald, Institute of Microbiology, Greifswald, Germany. sandra.maass@uni-greifswald.de.
Minia Antelo-VarelaDepartment of Microbial Proteomics, University of Greifswald, Institute of Microbiology, Greifswald, Germany.
Florian BonnDepartment of Microbial Proteomics, University of Greifswald, Institute of Microbiology, Greifswald, Germany.
Dörte BecherDepartment of Microbial Proteomics, University of Greifswald, Institute of Microbiology, Greifswald, Germany.
Universität Greifswald · DEUniversity of Basel · CH

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Absolute protein quantification is an essential tool for system biology approaches and elucidation of stoichiometry of multi-protein complexes. In this updated chapter, a universal protocol for gel-free absolute protein quantification in bacterial systems is described, which provides adapted methods for cytosolic and membrane proteins. This protocol can be used for sample preparation prior to miscellaneous mass spectrometry-based quantification workflows like AQUA, Hi3, and emPAI. In addition, a focus has been set to the specific challenges in antibiotic stress research.

Indexed as

Anti-Bacterial AgentsBacterial ProteinsMass SpectrometryMembrane ProteinsSpecimen HandlingAnti-Bacterial AgentsBacterial ProteinsMembrane ProteinsAbsolute protein quantificationAntibiotic stress responseCytosolGel free proteomicsIn-solution digestMass spectrometryMembraneSample preparationS-trap

Identifiers

PMID36445593
OpenAlexW4310346463

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.