Evidence map›Paper›PMID 36445586›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Tracking Global and Local Changes in Membrane Fluidity Through Fluorescence Spectroscopy and Microscopy.

Madeleine Humphrey, Ireny Abdelmesseh Nekhala, Kathi Scheinpflug, Oxana Krylova, Ann-Britt Schäfer, Jessica A Buttress, Michaela Wenzel, Henrik Strahl

Abstract read
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Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Lipid Matters: How Herbicidal Ionic Liquids Engage with Membranes.The journal of physical chemistry. B · 2026
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Madeleine HumphreyCentre for Bacterial Cell Biology, Biosciences Institute, Newcastle University, Newcastle upon Tyne, UK.
Ireny Abdelmesseh NekhalaDivision of Chemical Biology, Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden.
Kathi ScheinpflugDepartment of Chemical Biology, Leibniz-Institut für Molekulare Pharmakologie (FMP), Berlin, Germany.
Oxana KrylovaDepartment of Chemical Biology, Leibniz-Institut für Molekulare Pharmakologie (FMP), Berlin, Germany.
Ann-Britt SchäferDivision of Chemical Biology, Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden.
Jessica A ButtressCentre for Bacterial Cell Biology, Biosciences Institute, Newcastle University, Newcastle upon Tyne, UK.
Michaela WenzelDivision of Chemical Biology, Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, Sweden. wenzelm@chalmers.se.
Henrik StrahlCentre for Bacterial Cell Biology, Biosciences Institute, Newcastle University, Newcastle upon Tyne, UK. h.strahl@ncl.ac.uk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Membrane fluidity is a critical parameter of cellular membranes, which cells continuously strive to maintain within a viable range. Interference with the correct membrane fluidity state can strongly inhibit cell function. Triggered changes in membrane fluidity and associated impacts on lipid domains have been postulated to contribute to the mechanism of action of membrane targeting antimicrobials, but the corresponding analyses have been hampered by the absence of readily available analytical tools. Here, we expand upon the protocols outlined in the first edition of this book, providing further and alternative protocols that can be used to measure changes in membrane fluidity. We provide detailed protocols, which allow straightforward in vivo and in vitro measurement of antibiotic compound-triggered changes in membrane fluidity and fluid membrane microdomains. Furthermore, we summarize useful strains constructed by us and others to characterize and confirm lipid specificity of membrane antimicrobials directly in vivo.

Indexed as

Membrane FluidityMicroscopyCell MembraneLipidsSpectrometry, FluorescenceLipidsDiIC12Fatty acid disorderLaurdanLipid domainsLipid mutantsLipid packingMembrane fluidityMembrane microdomainsMembrane targeting antimicrobialsNile RedRIFs

Identifiers

PMID36445586

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.