ArticleMethods in molecular biology (Clifton, N.J.)2023
A Whole-Cell Assay for Detection of Antibacterial Activity in Actinomycete Culture Supernatants.
Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
1 citing paper in PubMed, 1 citations in OpenAlex.
- Overview on Strategies and Assays for Antibiotic Discovery.Pharmaceuticals (Basel, Switzerland) · 2022Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Whole-cell antibacterial assays are particularly suitable for fast detection and semi-quantification of bioactivities in extracts or other solutions such as microbial culture supernatants. As Actinomycetales, including the members of the genus Streptomyces, are one of the most potent "suppliers" of antibiotics and other bioactive compounds, there is a strong interest in the development of useful assays enabling early identification of such valuable producers. Furthermore, such assays facilitate the screening of a large collection of clones for the detection of engineered "super-producers" that are essential for industrial manufacturing of the respective product.In this protocol, we describe a whole-cell assay for a fast detection of antimicrobial agents in culture supernatants. As model, Streptomyces fradiae DSM 41546, the producer of the antibiotic tylosin, and the test strain Bacillus subtilis ATCC 6051 are used. Culture supernatants of S. fradiae DSM 41546 and controls are diluted, and their antibiotic activity is tested against B. subtilis ATCC 6051. For the dilutions of tylosin-containing culture supernatant, a clear concentration-dependent growth inhibition effect on B. subtilis ATCC 6051 is demonstrated. In contrast, dilutions of the culture supernatant lacking tylosin do not inhibit the growth of B. subtilis. Moreover, including defined concentrations of tylosin facilitates the semi-quantification of tylosin in the culture supernatants.Our results confirm the applicability of the assay in fast screenings for antimicrobial products in culture supernatants. The protocol can be used as positive control within screening campaigns in the drug discovery field.
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Registered trials
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