ArticlemBio2022
Within-Host Genotypic and Phenotypic Diversity of Contemporaneous Carbapenem-Resistant Klebsiella pneumoniae from Blood Cultures of Patients with Bacteremia.
Article in mBio, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Investigating Bacillus anthracis genomic diversity and trait-specific lineages in an endemic area in northern Tanzania through a combination of traditional and culture-free sequencing approaches.PLoS neglected tropical diseases · 2026Article
- Loss of O-antigen due toScience advances · 2026Article
- Blood Cultures Contain Populations of Genetically Diverse Candida albicans Strains that May Differ in Echinocandin Tolerance and Fitness.The Journal of infectious diseases · 2026Article
- The dual threat of hypervirulent and multidrug-resistantFrontiers in microbiology · 2026Review
- InvestigatingbioRxiv : the preprint server for biology · 2025Article
- Genomic and Phenotypic Characterization of Two High-RiskAntibiotics (Basel, Switzerland) · 2025Article
- Aneuploidy, polyploidy, and loss of heterozygosity distinguish serial bloodstream isolates ofbioRxiv : the preprint server for biology · 2025Article
- Parallel within-host evolution alters virulence factors in an opportunistic Klebsiella pneumoniae during a hospital outbreak.Nature communications · 2025Article
- Molecular and virulence differences ofFrontiers in microbiology · 2025Article
- Detection and molecular characterization of carbapenem-resistant gram-negative bacterial isolates.AMB Express · 2024Article
- Comparison of Multiple Carbapenemase Tests Based on an Unbiased Colony-Selection Method.Biomedicines · 2024Article
- Progress and New Challenges in Combatting the Threat of Antimicrobial Resistance: Perspective From an Infectious Diseases Pharmacist.The Journal of infectious diseases · 2024Article
- Genotypic diversity and unrecognized antifungal resistance among populations of Candida glabrata from positive blood cultures.Nature communications · 2023Article
- Genotypic diversity and unrecognized antifungal resistance among populations of Candida glabrata from positive blood cultures.Research square · 2023Article
- Quantifying patient- and hospital-level antimicrobial resistance dynamics inmedRxiv : the preprint server for health sciences · 2023Article
- Molecular and Clinical Characteristics of Carbapenem-ResistantInfection and drug resistance · 2023Article
Corrections and comments
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Authors and funding
13 authors.
Funding
Abstract
It is unknown whether bacterial bloodstream infections (BSIs) are commonly caused by single organisms or mixed microbial populations. We hypothesized that contemporaneous carbapenem-resistant Klebsiella pneumoniae (CRKP) strains from blood cultures of individual patients are genetically and phenotypically distinct. We determined short-read whole-genome sequences of 10 sequence type 258 (ST258) CRKP strains from blood cultures in each of 6 patients (Illumina HiSeq). Strains clustered by patient by core genome and pan-genome phylogeny. In 5 patients, there was within-host strain diversity by gene mutations, presence/absence of antibiotic resistance or virulence genes, and/or plasmid content. Accessory gene phylogeny revealed strain diversity in all 6 patients. Strains from 3 patients underwent long-read sequencing for genome completion (Oxford Nanopore) and phenotypic testing. Genetically distinct strains within individuals exhibited significant differences in carbapenem and other antibiotic responses, capsular polysaccharide (CPS) production, mucoviscosity, and/or serum killing. In 2 patients, strains differed significantly in virulence during mouse BSIs. Genetic or phenotypic diversity was not observed among strains recovered from blood culture bottles seeded with index strains from the 3 patients and incubated
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