ArticleBMC biotechnology2022
Development of a microbial protease for composting swine carcasses, optimization of its production and elucidation of its catalytic hydrolysis mechanism.
Article in BMC biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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5 citing papers in PubMed, 9 citations in OpenAlex.
- Designing a Multi-Epitope Vaccine Candidate AgainstVeterinary sciences · 2026Article
- Partial Purification, Characterisation and Gene Detection of Protease byTropical life sciences research · 2025Article
- Screening of Bovine Coronavirus Multiepitope Vaccine Candidates: An Immunoinformatics Approach.Transboundary and emerging diseases · 2024Article
- Preparation, purification, and biochemical of fat-degrading bacterial enzymes from pig carcass compost and its application.BMC biotechnology · 2023Article
- Microbial proteases and their applications.Frontiers in microbiology · 2023Review
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundDead swine carcass composting is an excellent method for harmless treatment and resource utilization of swine carcass. However, poor biodegradation ability of traditional composting results in poor harmless treatment effect. Researches report that the biodegradation ability of composting can be improved by inoculation with enzyme-producing microorganisms or by inoculation with enzyme preparations. At present, the researches on improving the efficiency of dead swine carcass composting by inoculating enzyme-producing microorganisms have been reported. However, no work has been reported on the development of enzyme preparations for dead swine carcass composting. METHODOLOGY: The protease-producing strain was isolated by casein medium, and was identified by 16 S rRNA gene sequencing. The optimal fermentation conditions for maximum protease production were gradually optimized by single factor test. The extracellular protease was purified by ammonium sulfate precipitation and Sephadex G-75 gel exclusion chromatography. The potential for composting applications of the purified protease was evaluated by characterization of its biochemical properties. And based on amino acid sequence analysis, molecular docking and inhibition test, the catalytic hydrolysis mechanism of the purified protease was elucidated.
resultsIn this study, a microbial protease was developed for swine carcass composting. A protease-producing strain DB1 was isolated from swine carcass compositing and identified as Serratia marcescen. Optimum fermentation conditions for maximum protease production were 5 g/L glucose, 5 g/L urea, 1.5 mmol/L Mg
conclusionsIn general, the microbial protease developed in this study was suitable for industrial production and has the potential to enhance composting at thermophilic stage. Moreover, the catalytic hydrolysis mechanism of the protease was further analyzed in this study.
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