ArticleNature methods2023
A three-photon head-mounted microscope for imaging all layers of visual cortex in freely moving mice.
Article in Nature methods, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed, 84 citations in OpenAlex.
- Miniscope Zero: a fully wireless, single-cell-resolution miniature microscope for imaging neural dynamics in freely behaving animals.bioRxiv : the preprint server for biology · 2026Article
- Simultaneous two- and three-photon multiplane imaging across cortical layers in freely moving mice.Nature methods · 2026Article
- Multifiber Array-Based Photometry System for Multiregional Functional Mapping in the Mouse Brain.The European journal of neuroscience · 2026Article
- The neuro-skeletal crosstalk: Mechanisms, clinical implications, and smart material interventions.Journal of orthopaedic translation · 2026Review
- 2P-FENDO-II: A fiber bundle microscope for all-optical, large field-of-view brain studies in freely moving mice.Cell reports methods · 2026Article
- Miniaturized widefield microscope for high speed in vivo voltage imaging.Biomedical optics express · 2026Article
- High-speed neural imaging with multiplexed miniaturized two-photon microscopy.Cell reports methods · 2025Article
- High-throughput two-photon volumetric brain imaging in freely moving mice.Nature communications · 2025Article
- Evaluating the outcomes of pluripotent stem-cell-derived photoreceptor transplantation in retinal repair.The FEBS journal · 2025Review
- Tissue type and tissue depth dependency of pulse broadening in three-photon microscopy: impact on third harmonic generation.Biomedical optics express · 2025Article
- A versatile miniature two-photon microscope enabling multicolor deep-brain imaging.Nature methods · 2025Article
- Three-photon microscopy: an emerging technique for deep intravital brain imaging.Nature reviews. Neuroscience · 2025Review
- State-of-the-art preclinical techniques to study the impact of spreading depolarizations in awake rodents.The journal of headache and pain · 2025Review
- Miniaturized widefield microscope for high speed in vivo voltage imaging.bioRxiv : the preprint server for biology · 2025Article
- Open-source, high performance miniature 2-photon microscopy systems for freely behaving animals.Nature communications · 2025Article
- Multiphoton Neurophotonics: Recent Advances in Imaging and Manipulating Neuronal Circuits.ACS photonics · 2025Review
- MiniXL: An open-source, large field-of-view epifluorescence miniscope enabling single-cell resolution and multi-region imaging in mice.Science advances · 2025Article
- The role of electroencephalography in epilepsy research-From seizures to interictal activity and comorbidities.Epilepsia · 2025Review
- Wide-field fluorescence navigation system for efficient miniature multiphoton imaging in freely behaving animals.Neurophotonics · 2025Article
- ONIX: a unified open-source platform for multimodal neural recording and perturbation during naturalistic behavior.Nature methods · 2025Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Advances in head-mounted microscopes have enabled imaging of neuronal activity using genetic tools in freely moving mice but these microscopes are restricted to recording in minimally lit arenas and imaging upper cortical layers. Here we built a 2-g, three-photon excitation-based microscope, containing a z-drive that enabled access to all cortical layers while mice freely behaved in a fully lit environment. The microscope had on-board photon detectors, robust to environmental light, and the arena lighting was timed to the end of each line-scan, enabling functional imaging of activity from cortical layer 4 and layer 6 neurons expressing jGCaMP7f in mice roaming a fully lit or dark arena. By comparing the neuronal activity measured from populations in these layers we show that activity in cortical layer 4 and layer 6 is differentially modulated by lit and dark conditions during free exploration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.