Evidence map›Paper›PMID 36441333›Full record

ArticleVirus genes2023

Full-genome characterisation of a putative novel serotype of Yonaguni orbivirus isolated from cattle in Yunnan province, China.

Zhenxing Yang, Yuwen He, Jinxin Meng, Nan Li, Jinglin Wang

Abstract read
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In one paragraph

Article in Virus genes, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Zhenxing Yang *Yunnan Tropical and Subtropical Animal Viral Disease Laboratory, Key Laboratory of Transboundary Animal Diseases Prevention and Control (Co-construction by Ministry and Province), Yunnan Animal Science and Veterinary Institute, Kunming, Yunnan Province, China.
Yuwen He *Yunnan Tropical and Subtropical Animal Viral Disease Laboratory, Key Laboratory of Transboundary Animal Diseases Prevention and Control (Co-construction by Ministry and Province), Yunnan Animal Science and Veterinary Institute, Kunming, Yunnan Province, China.
Jinxin MengYunnan Tropical and Subtropical Animal Viral Disease Laboratory, Key Laboratory of Transboundary Animal Diseases Prevention and Control (Co-construction by Ministry and Province), Yunnan Animal Science and Veterinary Institute, Kunming, Yunnan Province, China.
Nan LiYunnan Tropical and Subtropical Animal Viral Disease Laboratory, Key Laboratory of Transboundary Animal Diseases Prevention and Control (Co-construction by Ministry and Province), Yunnan Animal Science and Veterinary Institute, Kunming, Yunnan Province, China.
Jinglin WangYunnan Tropical and Subtropical Animal Viral Disease Laboratory, Key Laboratory of Transboundary Animal Diseases Prevention and Control (Co-construction by Ministry and Province), Yunnan Animal Science and Veterinary Institute, Kunming, Yunnan Province, China. Wangjl107@163.com.

Funding

Basic Research Projects of Yunnan Province 2019FA015, 202201AS070062Independent research project of Yunnan Tropical and Subtropical Animal Viral Disease Laboratory 2022RW001Projects funded by the central government to guide local scientific and Technological Development 202207AB110006Yunnan Chenggong expert workstation 202005AF150034Yunnan Science and Technology Talents and Platform Plan 2018HB046
6 · The paper itself

Abstract

In July 2019, a novel viral strain (JH2019C603) was isolated from sentinel cattle in Jinghong City, in the subtropical region of Yunnan Province, China. The virus replicated and caused cytopathological effects in both Aedes albopictus (C6/36) and Baby Hamster Syrian Kidney (BHK-21) cells. Agarose gel electrophoresis analysis revealed a viral genome comprised of 10 segments of double-stranded RNA, with a 1-2-2-1-1-1-1-1 migration pattern. Complete genome sequences of the JH2019C603 virus were determined through full-length cDNA amplification. Phylogenetic analysis based on the amino acid (aa) sequences of RNA-dependent RNA Polymerase (Pol), Major subcore (T2) and Major core-surface (T13) showed that JH2019C603 clustered with Yonaguni orbivirus (YONOV) from Japan, with aa identities relative to YONOV of 97.7% (Pol), 99.0% (T2) and 98.5% (T13). However, phylogenetic analysis based on the aa sequences of the outer capsid protein one and two (OC1 and OC2) showed that JH2019C603 formed an independent branch in the phylogenetic tree, and its aa identity with YONOV was only 55.4% (OC1) and 80.8% (OC2), respectively. Compared with the prototype of YONOV, a notable sequence deletion was observed in the 3' non-coding region of NS1, with the NS1 of JH2019C603 encoded within segment 7 (Seg-7), in contrast to YONOV, which contains NS1 in Seg-6. These results indicate that JH2019C603 belongs to the YONOV lineage and might be a novel serotype or a highly variant strain of YONOV. These findings will facilitate the identification of new isolates and clarify their geographical distribution, epidemiology, genetic diversity and possible disease associations.

Indexed as

OrbivirusAmino Acid SequenceAnimalsCattleChinaCricetinaeGenome, ViralPhylogenyRNA, ViralSerogroupRNA, ViralChinaFull genomic analysisInfection in CattleVirus isolationYonaguni orbivirus

Identifiers

PMID36441333

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.