Evidence map›Paper›PMID 36440965›Full record

ArticleBiology of reproduction2023

SWI/SNF chromatin remodeling subunit Smarca4/BRG1 is essential for female fertility†.

Atefeh Abedini, David A Landry, Angus D Macaulay, Het Vaishnav, Ashna Parbhakar, Dalia Ibrahim, Reza Salehi, Vincent Maranda, Elizabeth Macdonald, Barbara C Vanderhyden

Erratum issuedOpen access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biology of reproduction, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.8field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Article
  6. Article
  7. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Atefeh AbediniDepartment of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada.
David A LandryDepartment of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada.
Angus D MacaulayChronic Diseases Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada.
Het VaishnavDepartment of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada.
Ashna ParbhakarCancer Therapeutics Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada.
Dalia IbrahimDepartment of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada.
Reza SalehiChronic Diseases Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada.
Vincent MarandaDepartment of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada.
Elizabeth MacdonaldDepartment of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada.
Barbara C VanderhydenDepartment of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada.
Ottawa Hospital · CAOttawa Hospital Research Institute

Funding

Canadian Rare Disease Models and Mechanisms Network and the Cancer Research SocietyNatural Sciences and Engineering Research Council of Canada
6 · The paper itself

Abstract

Mammalian folliculogenesis is a complex process that involves the regulation of chromatin structure for gene expression and oocyte meiotic resumption. The SWI/SNF complex is a chromatin remodeler using either Brahma-regulated gene 1 (BRG1) or BRM (encoded by Smarca4 and Smarca2, respectively) as its catalytic subunit. SMARCA4 loss of expression is associated with a rare type of ovarian cancer; however, its function during folliculogenesis remains poorly understood. In this study, we describe the phenotype of BRG1 mutant mice to better understand its role in female fertility. Although no tumor emerged from BRG1 mutant mice, conditional depletion of Brg1 in the granulosa cells (GCs) of Brg1fl/fl;Amhr2-Cre mice caused sterility, whereas conditional depletion of Brg1 in the oocytes of Brg1fl/fl;Gdf9-Cre mice resulted in subfertility. Recovery of cumulus-oocyte complexes after natural mating or superovulation showed no significant difference in the Brg1fl/fl;Amhr2-Cre mutant mice and significantly fewer oocytes in the Brg1fl/fl;Gdf9-Cre mutant mice compared with controls, which may account for the subfertility. Interestingly, the evaluation of oocyte developmental competence by in vitro culture of retrieved two-cell embryos indicated that oocytes originating from the Brg1fl/fl;Amhr2-Cre mice did not reach the blastocyst stage and had higher rates of mitotic defects, including micronuclei. Together, these results indicate that BRG1 plays an important role in female fertility by regulating granulosa and oocyte functions during follicle growth and is needed for the acquisition of oocyte developmental competence.

Indexed as

ChromatinNeoplasmsAnimalsChromatin Assembly and DisassemblyDNA HelicasesFemaleFertilityMammalsMiceNuclear ProteinsTranscription FactorsChromatinDNA HelicasesNuclear ProteinsSmarca4 protein, mouseTranscription Factorsembryosgranulosa cellsinfertilityoocyteoocyte qualitySWI/SNF complex

Identifiers

PMID36440965
PMCPMC9930400
OpenAlexW4310076070

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.