Evidence map›Paper›PMID 36437439›Full record

ArticleMolecular biotechnology2023

Establishment of Transcriptional Gene Silencing Targeting the Promoter Regions of GFP, PDS, and PSY Genes in Cotton using Virus-Induced Gene Silencing.

Aqsa Hafeez Khan, Afzal Akram, Muhammad Saeed, Mehboob Ur Rahman, Atiq Ur Rehman, Shahid Mansoor, Imran Amin

Abstract read
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In one paragraph

Article in Molecular biotechnology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
2.3field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Aqsa Hafeez KhanNational Institute for Biotechnology and Genetic Engineering (NIBGE), College of Pakistan Institute of Engineering and Applied Sciences, Nilore, Islamabad, Pakistan.
Afzal AkramNational Institute for Biotechnology and Genetic Engineering (NIBGE), College of Pakistan Institute of Engineering and Applied Sciences, Nilore, Islamabad, Pakistan.
Muhammad SaeedNational Institute for Biotechnology and Genetic Engineering (NIBGE), College of Pakistan Institute of Engineering and Applied Sciences, Nilore, Islamabad, Pakistan.
Mehboob Ur RahmanNational Institute for Biotechnology and Genetic Engineering (NIBGE), College of Pakistan Institute of Engineering and Applied Sciences, Nilore, Islamabad, Pakistan.
Atiq Ur RehmanNational Institute for Biotechnology and Genetic Engineering (NIBGE), College of Pakistan Institute of Engineering and Applied Sciences, Nilore, Islamabad, Pakistan.
Shahid MansoorNational Institute for Biotechnology and Genetic Engineering (NIBGE), College of Pakistan Institute of Engineering and Applied Sciences, Nilore, Islamabad, Pakistan.
Imran AminNational Institute for Biotechnology and Genetic Engineering (NIBGE), College of Pakistan Institute of Engineering and Applied Sciences, Nilore, Islamabad, Pakistan. imranamin1@yahoo.com.ORCID http://orcid.org/0000-0003-3063-4103
National Institute for Biotechnology and Genetic Engineering · PK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Virus-induced gene silencing (VIGS) by deploying viral-based vectors such as tobacco rattle virus (TRV) is a homology-based gene silencing technique in post-transcriptional gene silencing (PTGS) and transcriptional gene silencing (TGS) to validate the function of particular genes. The study presented here showed the induction of DNA methylation in the promoter regions of three phenotypic marker genes in different cotton accessions, including two endogenous genes such as phytoene desaturase (PDS) and phytoene synthase (PSY), and an exogenous gene, such as green fluorescent protein (GFP). First, DNA methylation was established in transgenic GFP cotton where methylation persisted up to S

Indexed as

Gene SilencingPlant VirusesDNA MethylationGene Expression Regulation, PlantGenetic VectorsGreen Fluorescent ProteinsNicotianaOxidoreductasesPromoter Regions, GeneticGreen Fluorescent ProteinsOxidoreductasesphytoene dehydrogenaseGossypiumPhytoene synthaseTobacco rattle virus vectorTranscriptional gene silencingVirus-induced transcriptional gene silencing

Identifiers

PMID36437439
OpenAlexW4310276445

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.