Evidence map›Paper›PMID 36434260›Full record

ArticleFolia microbiologica2023

Deletion of the cheZ gene results in the loss of swimming ability and the decrease of adhesion ability to Caco-2 cells in Escherichia coli Nissle 1917.

Bingming Ou, Haihui Lv, Hua Ge, Dexiang Fu, Xin Lin, Saiqiao Huang, Xiaojie Chen, Yufu Liu, Sainan Li, Wenhua Liu and 3 more

Abstract read
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In one paragraph

Article in Folia microbiologica, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Bingming OuSchool of Life Sciences, Zhaoqing University, Zhaoqing, 526061, China.
Haihui LvSchool of Life Sciences, Zhaoqing University, Zhaoqing, 526061, China.
Hua GeSchool of Life Sciences, Zhaoqing University, Zhaoqing, 526061, China.
Dexiang FuSchool of Life Sciences, Zhaoqing University, Zhaoqing, 526061, China.
Xin LinSchool of Life Sciences, Zhaoqing University, Zhaoqing, 526061, China.
Saiqiao HuangSchool of Life Sciences, Zhaoqing University, Zhaoqing, 526061, China.
Xiaojie ChenSchool of Life Sciences, Zhaoqing University, Zhaoqing, 526061, China.
Yufu LiuSchool of Life Sciences, Zhaoqing University, Zhaoqing, 526061, China.
Sainan LiSchool of Life Sciences, Zhaoqing University, Zhaoqing, 526061, China.
Wenhua LiuSchool of Life Sciences, Zhaoqing University, Zhaoqing, 526061, China.
Lihua HuangSchool of Life Sciences, Zhaoqing University, Zhaoqing, 526061, China.
Ying YangCollege of Animal Science, Guizhou University, Guiyang, 550025, China. yang_ying510@163.com.
Minyu ZhangSchool of Life Sciences, Zhaoqing University, Zhaoqing, 526061, China. zhangminyu_zmy@126.com.ORCID http://orcid.org/0000-0003-2326-831X

Funding

Basic and Applied Basic Research Joint Fund of Guangdong Province 2019A1515111186College Students' Innovation and Entrepreneurship Training Plan Program of Guangdong Province S202010580051College Students' Innovation and Entrepreneurship Training Plan Program of Guangdong Province S202210580052Modern Agricultural Technology Industry System of Guizhou province ControlModern Agricultural Technology Industry System of Guizhou province Functional laboratory of Disease PreventionNational Natural Science Foundation of China Grant No. 32102703Science and Technology Support Plan for Youth Innovation of Colleges and Universities of Guangdong Province of China 202110580008Scientific Research Start-up Fund of Zhaoqing University 611/180160
6 · The paper itself

Abstract

The aim of this study was to elucidate the biological functions of the motility regulatory protein CheZ in the probiotic strain Escherichia coli Nissle 1917. A cheZ gene deletion strain Nissle 1917ΔcheZ was constructed using the CRISPR/Cas9 two-plasmid system, and the corresponding complemented strain Nissle 1917ΔcheZ/pBR322-cheZ was established. Combined studies of growth kinetics testing, motility assays, swarming motility assays, and bacterial adherence assays were performed to study the motility regulatory protein CheZ-mediated functions in the prototype Nissle 1917 strain, its isogenic cheZ mutant, and the corresponding complemented strain. The growth rate of the cheZ mutant strain was lower than that of the wild-type strain in the exponential growth phase. The motility of the cheZ mutant strain was significantly lower than that of the wild-type strain. And the adhesion ability of ΔcheZ mutant to the Caco-2 cells was significantly lower than that of the wild-type strain and complemented strain. In conclusion, the results presented in our study suggested that the deletion of the cheZ gene in E. coli Nissle 1917 led to a significant reduction of its swimming ability and a subsequent marked decrease of adhesion to the Caco-2 cells.

Indexed as

Escherichia coliProbioticsCaco-2 CellsGene DeletionHumansSwimmingAdherenceCheZGrowth kineticsNissle 1917Swarming motility

Identifiers

PMID36434260

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.