Evidence map›Paper›PMID 36430927›Full record

ArticleInternational journal of molecular sciences2022

Molecular Characterization of a B Cell Adaptor for Phosphoinositide 3-Kinase Homolog in Lamprey (

Mengqi Chai, Xiujia Liu, Lin Wei, Jun Li, Meng Gou, Ting Zhu, Yinglun Han, Xin Liu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 53% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Updating Our Knowledge on Fish Immunology.International journal of molecular sciences · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Mengqi ChaiLamprey Research Center, College of Life Science, Liaoning Normal University, Dalian 116081, China.
Xiujia LiuLamprey Research Center, College of Life Science, Liaoning Normal University, Dalian 116081, China.
Lin WeiLamprey Research Center, College of Life Science, Liaoning Normal University, Dalian 116081, China.
Jun LiLamprey Research Center, College of Life Science, Liaoning Normal University, Dalian 116081, China.
Meng GouLamprey Research Center, College of Life Science, Liaoning Normal University, Dalian 116081, China.
Ting ZhuLamprey Research Center, College of Life Science, Liaoning Normal University, Dalian 116081, China.
Yinglun HanLamprey Research Center, College of Life Science, Liaoning Normal University, Dalian 116081, China.
Xin LiuLamprey Research Center, College of Life Science, Liaoning Normal University, Dalian 116081, China.ORCID 0000-0003-1597-0096
Liaoning Normal University · CN

Funding

High-level Talent Innovation Support Program of Dalian 2019RQ046High-level Talent Innovation Support Program of Dalian 2020RQ043High-level Talent Innovation Support Program of Dalian 2020RQ071Liaoning Revitalization Talents Prograrn XLYC2007189National Natural Science Foundation of Liaoning Province 2020-MS-245Project of the Educational Department of Liaoning Province China LJKZ0990Project of the Educational Department of Liaoning Province China LQ2020025
6 · The paper itself

Abstract

Human B cell adaptor for phosphoinositide 3-kinase (BCAP) is identified as an adaptor protein expressed in B cells and plays a critical immunomodulatory role in B cell receptor signaling and humoral immune response. In the current study, a homolog of BCAP (Lja-BCAP) was identified in Lampetra japonica. The open reading frame of Lja-BCAP contains 2181bp nucleotides and encodes a protein of 726 amino acids. After being stimulated by mixed bacteria, the mRNA and protein expression levels of Lja-BCAP and the activation levels of tyrosine kinases increased significantly in peripheral blood lymphocytes, gills and supraneural myeloid bodies, respectively. However, after the knockdown of Lja-BCAP by RNAi in vivo, the activation of tyrosine kinases was inhibited in the above tissues, which indicated that Lja-BCAP participated in the anti-bacterial immune response of lampreys. After lipopolysaccharide (LPS) stimulation, the expression of Lja-BCAP in peripheral blood lymphocytes, gills and supraneural myeloid bodies were significantly up-regulated 2.5, 2.2, and 11.1 times (p < 0.05) compared to the control group, respectively; while after phytohemagglutinin (PHA) stimulation, the up-regulation of Lja-BCAP was only detected in peripheral blood lymphocytes. The above results show that Lja-BCAP mainly participates in the LPS-mediated immune response of lampreys.

Indexed as

LampreysPhosphatidylinositol 3-KinasesAnimalsHumansImmunityLipopolysaccharidesPhosphatidylinositol 3-KinaseProtein-Tyrosine KinasesTyrosineLipopolysaccharidesPhosphatidylinositol 3-KinasePhosphatidylinositol 3-KinasesProtein-Tyrosine KinasesTyrosineBCAPimmune responseLampetra japonicaLipopolysaccharidelymphocyte

Identifiers

PMID36430927
PMCPMC9695028
OpenAlexW4309741905

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.