Evidence map›Paper›PMID 36430882›Full record

ArticleInternational journal of molecular sciences2022

Modular Site-Specific Conjugation of Nanobodies Using Two Co-Associating Tags.

Eric Moeglin, Lina Barret, Bruno Chatton, Mariel Donzeau

Abstract read
In one paragraph

Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. A Rapid and Modular Nanobody Assay for Plug-and-Play Antigen Detection.bioRxiv : the preprint server for biology · 2025
    Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Eric MoeglinUMR7242 Biotechnologie et Signalisation Cellulaire, Université de Strasbourg, F-67412 Illkirch, France.ORCID 0000-0001-9886-2247
Lina BarretIMPReSs Facility, Biotechnology and Cell Signaling, CNRS, University of Strasbourg, F-67412 Illkirch, France.
Bruno ChattonUMR7242 Biotechnologie et Signalisation Cellulaire, Université de Strasbourg, F-67412 Illkirch, France.ORCID 0000-0003-4515-7119
Mariel DonzeauUMR7242 Biotechnologie et Signalisation Cellulaire, Université de Strasbourg, F-67412 Illkirch, France.ORCID 0000-0002-1145-9218

Funding

ANR-SFRI ANR-20-SFRI-0012ITI Innovec (IdEx) ANR-10-IDEX-0002the Fondation ARC pour la recherche sur le cancer ARCthe Ligue Régionale contre le Cancer (CCIR-GE) CCIR-GE
6 · The paper itself

Abstract

The homogeneous labeling of antibodies and their fragments is a critical step for the generation of robust probes used in immuno-detection applications. To date, numerous chemical, genetic and peptide-based site-specific coupling methods have been developed. Among these methods, co-assembling peptide-tags is one of the most straightforward and versatile solutions. Here, we describe site-specific labeling of nanobodies through the use of two co-associating peptides tags, E3 and K3, originating from the tetramerization domain of p53. These E3 and K3-tags provide a simple and robust method for associating stoichiometric amount of V

Indexed as

Single-Domain AntibodiesChemical PhenomenaHumansMolecular ProbesPeptidesProteinsMolecular ProbesPeptidesProteinsSingle-Domain Antibodiesconjugationnanobodysite-specificVHH

Identifiers

PMID36430882
PMCPMC9696751

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.