Evidence map›Paper›PMID 36430849›Full record

ArticleInternational journal of molecular sciences2022

Eukaryotic CRFK Cells Motion Characterized with Atomic Force Microscopy.

María Zamora-Ceballos, Juan Bárcena, Johann Mertens

Abstract read
In one paragraph

Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

María Zamora-CeballosCentro de Investigación en Sanidad Animal (CISA-INIA/CSIC), Valdeolmos, 28130 Madrid, Spain.
Juan BárcenaCentro de Investigación en Sanidad Animal (CISA-INIA/CSIC), Valdeolmos, 28130 Madrid, Spain.ORCID 0000-0001-6050-0654
Johann MertensMadrid Institute for Advanced Studies in Nanoscience (IMDEA Nanoscience), Campus de Cantoblanco, 28049 Madrid, Spain.

Funding

Ministry of Economy, Industry and Competitiveness PGC2018-099713-B-100Ministry of Science and Innovation BES-2017-081188Ministry of Science and Innovation PID2019-107145RB-100
6 · The paper itself

Abstract

We performed a time-lapse imaging with atomic force microscopy (AFM) of the motion of eukaryotic CRFK (Crandell-Rees Feline Kidney) cells adhered onto a glass surface and anchored to other cells in culture medium at 37 °C. The main finding is a gradient in the spring constant of the actomyosin cortex along the cells axis. The rigidity increases at the rear of the cells during motion. This observation as well as a dramatic decrease of the volume suggests that cells may organize a dissymmetry in the skeleton network to expulse water and drive actively the rear edge.

Indexed as

Eukaryotic CellsKidneyAnimalsCatsCell LineEpithelial CellsMicroscopy, Atomic Forceatomic force microscopycytoskeletoneukaryotic cellsmechanical propertiesmigrationmotionspring constanttopography

Identifiers

PMID36430849
PMCPMC9692694

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.