Evidence map›Paper›PMID 36430847›Full record

ArticleInternational journal of molecular sciences2022

Evaluation of Reference Genes for Real-Time Quantitative PCR Analysis in Tissues from Bumble Bees (

Kathannan Sankar, Hyung Joo Yoon, Young Bo Lee, Kyeong Yong Lee

Open access · goldAbstract read
In one paragraph

Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.4field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 4 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Kathannan SankarDivision of Apiculture, Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development of Administration, Wanju-gun 55365, Republic of Korea.
Hyung Joo YoonDivision of Apiculture, Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development of Administration, Wanju-gun 55365, Republic of Korea.
Young Bo LeeDivision of Apiculture, Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development of Administration, Wanju-gun 55365, Republic of Korea.
Kyeong Yong LeeDivision of Apiculture, Department of Agricultural Biology, National Institute of Agricultural Science, Rural Development of Administration, Wanju-gun 55365, Republic of Korea.
Rural Development Administration · KR

Funding

Rural Development Administration PJ015953012022)
6 · The paper itself

Abstract

Bumble bees are important alternative pollinators and model insects due to their highly developed sociality and colony management. In order to better understand their molecular mechanisms, studies focusing on the genetic and molecular aspects of their development and behavior are needed. Although quantitative real-time polymerase chain reaction (qRT-PCR) can be used to quantify the relative expression of target genes, internal reference genes (which are stably expressed across different lines and tissues) must first be identified to ensure the accurate normalization of target genes. In order to contribute to molecular studies on bumble bees, we used

Indexed as

Glyceraldehyde-3-Phosphate DehydrogenasesPeptide Elongation Factor 1ActinsAnimalsBeesFemalePhospholipases A2Real-Time Polymerase Chain ReactionActinsGlyceraldehyde-3-Phosphate DehydrogenasesPeptide Elongation Factor 1Phospholipases A2bumble beegene expressionqRT-PCRreference geneRefFinder

Identifiers

PMID36430847
PMCPMC9692494
OpenAlexW4309683820

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.