ArticleInternational journal of molecular sciences2022
Mechanism of Phase Separation in Aqueous Two-Phase Systems.
Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Aqueous Two-Phase Bioinks for Discrete Packing and Compartmentalization of 3D Bioprinted Cells.Advanced healthcare materials · 2026Article
- Effects of Arginine on Hierarchical Protein Aggregation.The protein journal · 2026Article
- Biomolecular condensates-Prerequisites for anhydrobiosis?Protein science : a publication of the Protein Society · 2025Review
- Underestimated role of macromolecular crowding in bioengineeredMaterials today. Bio · 2025Article
- Effects of Inorganic Salts on Phase Separation in Aqueous Solutions of Poly(ethylene glycol).International journal of molecular sciences · 2025Article
- Application of the Thermal Analysis of Frozen Aqueous Solutions to Assess the Miscibility of Hyaluronic Acid and Polymers Used for Dissolving Microneedles.Pharmaceutics · 2024Article
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Authors and funding
6 authors.
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Abstract
Liquid-liquid phase separation underlies the formation of membrane-less organelles inside living cells. The mechanism of this process can be examined using simple aqueous mixtures of two or more solutes, which are able to phase separate at specific concentration thresholds. This work presents the first experimental evidence that mesoscopic changes precede visually detected macroscopic phase separation in aqueous mixtures of two polymers and a single polymer and salt. Dynamic light scattering (DLS) analysis indicates the formation of mesoscopic polymer agglomerates in these systems. These agglomerates increase in size with increasing polymer concentrations prior to visual phase separation. Such mesoscopic changes are paralleled by changes in water structure as evidenced by Attenuated Total Reflection-Fourier Transform Infrared (ATR-FTIR) spectroscopic analysis of OH-stretch bands. Through OH-stretch band analysis, we obtain quantitative estimates of the relative fractions of four subpopulations of water structures coexisting in aqueous solutions. These estimates indicate that abrupt changes in hydrogen bond arrangement take place at concentrations below the threshold of macroscopic phase separation. We used these experimental observations to develop a model of phase separation in aqueous media.
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