Evidence map›Paper›PMID 36429188›Full record

ArticleFoods (Basel, Switzerland)2022

pH-Regulated Strategy and Mechanism of Antibody Orientation on Magnetic Beads for Improving Capture Performance of

Fuying Kang, Yin Yang, Jingwen Li, Erning Chen, Tian Hong, Lulu Zhao, Meihong Du

Open access · goldAbstract read
In one paragraph

Article in Foods (Basel, Switzerland), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.5field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Fuying KangInstitute of Analysis and Testing, Beijing Academy of Science and Technology, Beijing Center of Physical and Chemical Analysis, Beijing 100094, China.
Yin YangBeijing Academy of Science and Technology, Beijing 100094, China.
Jingwen LiInstitute of Analysis and Testing, Beijing Academy of Science and Technology, Beijing Center of Physical and Chemical Analysis, Beijing 100094, China.
Erning ChenInstitute of Analysis and Testing, Beijing Academy of Science and Technology, Beijing Center of Physical and Chemical Analysis, Beijing 100094, China.
Tian HongInstitute of Analysis and Testing, Beijing Academy of Science and Technology, Beijing Center of Physical and Chemical Analysis, Beijing 100094, China.
Lulu ZhaoInstitute of Analysis and Testing, Beijing Academy of Science and Technology, Beijing Center of Physical and Chemical Analysis, Beijing 100094, China.
Meihong DuInstitute of Analysis and Testing, Beijing Academy of Science and Technology, Beijing Center of Physical and Chemical Analysis, Beijing 100094, China.
Beijing Academy of Science and Technology · CN

Funding

BJAST Budding Talent Program BGS202116High-level Innovation Team Program HIT202201National Natural Science Foundation of China 31972146
6 · The paper itself

Abstract

Immunomagnetic beads (IMBs) have been widely used to capture and isolate target pathogens from complex food samples. The orientation of the antibody immobilized on the surface of magnetic beads (MBs) is closely related to the effective recognition with an antigen. We put forward an available strategy to orient the antibody on the surface of MBs by changing the charged amino group ratio of the reactive amino groups at optimal pH value. Quantum dots labeling antigen assay, antigen-binding fragment (Fab) accessibility assay and lysine mimicking were used for the first time to skillfully illustrate the antibody orientation mechanism. This revealed that the positively charged ε-NH

Indexed as

antibody orientationcapture efficiencyfoodborne pathogenimmunomagnetic beadspH regulation

Identifiers

PMID36429188
PMCPMC9689862
OpenAlexW4309605539

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.