ArticleDental materials : official publication of the Academy of Dental Materials2022
Salivary esterases dramatically reduce biostability of dentin collagen treated with galloylated polyphenols.
Article in Dental materials : official publication of the Academy of Dental Materials, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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3 citing papers in PubMed, 2 citations in OpenAlex.
- Dual Mechanisms of pH-Dependent Tea Astringency: Binding and Metabolism of Polyphenols Revealed by In Vivo and In Vitro Oral Processing.Foods (Basel, Switzerland) · 2026Article
- Zymography as a Tool for Exploring Protease Activity in Dental Research.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Evaluation of Microbiological Susceptibility and Long-term Adhesive Properties to Dentin of Primers with Terminalia catappa Linn.The journal of adhesive dentistry · 2024Article
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Authors and funding
5 authors at 1 institution in 1 country.
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Abstract
objectiveTo investigate the effects of salivary esterases on biostability of collagen treated by galloylated polyphenols.
methodsHuman dentin was microtomed into 6-μm-thick films, which were demineralized and treated for 60 s using solutions containing 0.6% and 2% of one of the crosslinkers: tannic acid (TAC), epigallocatechin gallate (EGCG), epigallocatechin (EGC), and N-[3-dimethylaminopropyl]-N'-ethylcarbodiimide hydrochloride (EDC)/N-hydroxysuccinimide (NHS), and for 1 h using EDC/NHS. Half of the treated and untreated (control) films were subjected to human saliva incubation. Collagen biostability was assessed via exogenous protease biodegradation by weight loss and hydroxyproline release, and endogenous MMPs by in situ zymography. The degradation products of galloylated polyphenols (TAC and EGCG) by saliva were monitored using proton nuclear magnetic resonance (
resultsCollagen films treated with TAC and EGCG exhibited significantly improved biostability (p < 0.05); however, the enhanced biostability was severely reduced after saliva incubation (p < 0.001). For EDC/NHS treated collagen, saliva incubation showed negligible effect on the biostability. SIGNIFICANCE: The galloyl group plays important role in collagen crosslinking, inducing higher biostability. However, galloylated polyphenols crosslinked on collagen are highly susceptible to metabolism of human saliva by salivary esterase, dramatically compromising the enhanced biostability.
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