Evidence map›Paper›PMID 36427153›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

In Embryo Gene Reporter Assays for Evaluation of Cis-Regulatory Regions.

Carolina Purcell Goes, Tatiane Y Kanno, C Y Irene Yan

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.1field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 2 countries.

Carolina Purcell GoesDepartment of Cell and Developmental Biology, Biomedical Sciences Institute, Universidade de São Paulo, São Paulo, Brazil.
Tatiane Y KannoDepartment of Molecular Biology and Genetics, Cornell University, Ithaca, NY, USA.
C Y Irene YanDepartment of Cell and Developmental Biology, Biomedical Sciences Institute, Universidade de São Paulo, São Paulo, Brazil. ireneyan@usp.br.
Universidade de São Paulo · BRCornell University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Gene expression reporter assays measure the relevance of cis-regulatory elements and DNA-binding proteins in modulating transcriptional activity. Commonly, they are performed in cell lines. However, regulation of transcriptional activity during development is complex and dynamic, and not many cell lines reproduce the embryonic conditions. Thus, conclusions derived from cell line data provide limited information about embryonic development. On the other hand, one of the major hurdles for embryonic assays is delivering reporter plasmids in a tissue-specific manner. In this sense, the chick embryo is a good model system to perform these assays. Electroporation of chick embryos provides temporal and spatially controlled plasmid delivery. Further, it is a well-established, easy, and an economical procedure. Here, we describe in detail how to measure in the chick neural tube (1) enhancer activity with GFP, (2) enhancer activity with luciferase, and (3) 3'UTR activity with luciferase.

Indexed as

Embryo, MammalianRegulatory Sequences, Nucleic AcidAnimalsBiological AssayChick EmbryoDNA-Binding ProteinsEmbryonic DevelopmentFemaleDNA-Binding ProteinsCis-regulatory regionsIn vivo reporter assayLuciferaseRegulatory elementsReporter quantification

Identifiers

PMID36427153
OpenAlexW4309971163

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.