Evidence map›Paper›PMID 36427146›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Easy Hi-C: A Low-Input Method for Capturing Genome Organization.

Leina Lu, Fulai Jin

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Review
  3. Correlation of Redondovirus andInternational journal of molecular sciences · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Leina LuDepartment of Genetics and Genome Sciences, School of Medicine, Case Western Reserve University, Cleveland, OH, USA. lxl534@case.edu.
Fulai JinDepartment of Genetics and Genome Sciences, School of Medicine, Case Western Reserve University, Cleveland, OH, USA. fxj45@case.edu.

Funding

Robust mapping of chromatin loops from sparse or single cell Hi-C data with DeepLoopR01HG009658 · NHGRI · CASE WESTERN RESERVE UNIVERSITY · PI Fulai Jin · 2017 to 2026
$5.1M
Elucidating human beta cell transcriptional regulome with low-input genomic technologiesR01DK113185 · NIDDK · CASE WESTERN RESERVE UNIVERSITY · PI Yan Li · 2018 to 2026
$4.0M
STAG2 mutations and 3D genome organization in glioblastoma multiformeR01CA267872 · NCI · GEORGETOWN UNIVERSITY · PI Fulai Jin, TODD A WALDMAN · 2022 to 2026
$2.7M
Mapping heritable chromatin loop variants with allele-specific Hi-C analysisR01HG012384 · NHGRI · CASE WESTERN RESERVE UNIVERSITY · PI Alan D Attie, Fulai Jin · 2023 to 2026
$2.6M
Understanding the variation of induced β-cell differentiation.R01DK131437 · NIDDK · CASE WESTERN RESERVE UNIVERSITY · PI JIN, FULAI, LI, YAN · 2022 to 2025
$2.4M
Simultaneous mapping of somatic mosaicism and kb-resolution 3D genome in single cells.UG3NS132061 · NINDS · CASE WESTERN RESERVE UNIVERSITY · PI JIN, FULAI, LI, YAN · 2023 to 2024
$805k
NCI NIH HHS R01 CA267872NHGRI NIH HHS R01 HG009658NHGRI NIH HHS R01 HG012384NIDDK NIH HHS R01 DK131437NINDS NIH HHS UG3 NS132061
6 · The paper itself

Abstract

Chromosome conformation capture technology and its derivatives have been widely used to study genome organization. Among them, Hi-C (chromosome conformation capture coupling with high-throughput sequencing) is popular in dissecting chromatin architecture on the genome-wide level. However, the intrinsic limitations prevent its application when it comes to rare samples. Here, we present easy Hi-C, a biotin-free technology that dramatically reduces DNA loss and is suitable for low-input samples.

Indexed as

ChromosomesGenomeChromatinChromosome MappingHigh-Throughput Nucleotide SequencingChromatinChromatin architectureChromosome conformation captureDeep sequencingEasy Hi-CHi-CProximity ligationRe-linearizationRestriction enzyme digestionSelf-circularization

Identifiers

PMID36427146
PMCPMC10040274

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.