Evidence map›Paper›PMID 36427142›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

ChIP-Seq Protocol for In Vitro Cell Differentiation Systems.

Jacqueline Copeland, Fjodor Merkuri, Marcos Simoes-Costa

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Jacqueline CopelandDepartment of Molecular Biology and Genetics, Cornell University, Ithaca, NY, USA.
Fjodor MerkuriDepartment of Molecular Biology and Genetics, Cornell University, Ithaca, NY, USA.
Marcos Simoes-CostaDepartment of Molecular Biology and Genetics, Cornell University, Ithaca, NY, USA. simoescosta@cornell.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In vitro differentiation systems provide a flexible platform for understanding complex developmental processes. Here, we provide a comprehensive protocol for the preparation and analysis of ChIP-seq libraries for human-induced neural crest cells (hiNCCs) from human embryonic stem cells (hESCs). This workflow is aimed at identifying interactions between transcription factors and cis regulatory elements, which serve as useful assays in uncovering gene regulatory principles during development.

Indexed as

Chromatin Immunoprecipitation SequencingHuman Embryonic Stem CellsCell DifferentiationChromatin ImmunoprecipitationHumansRegulatory Sequences, Nucleic AcidChromatin immunoprecipitation sequencing (ChIP-seq)In vitro differentiationProtein–DNA interactions

Identifiers

PMID36427142

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.