Evidence map›Paper›PMID 36427021›Full record

ArticleChemical communications (Cambridge, England)2022

Enzyme self-label-bound ATTO700 in single-molecule and super-resolution microscopy.

Michael Trumpp, Anna Oliveras, Hannes Gonschior, Julia Ast, David J Hodson, Petra Knaus, Martin Lehmann, Melissa Birol, Johannes Broichhagen

Open access · hybridAbstract read
In one paragraph

Article in Chemical communications (Cambridge, England), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
2.1field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 10 citations in OpenAlex.

  1. A Universal Protein Ladder for Standardization of Diverse FRET Assays.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Article
  2. Article
  3. Review
  4. Article
  5. Super-resolution imaging with a cucurbituril-encapsulated fluorophore.Chemical communications (Cambridge, England) · 2024
    Article
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 2 countries.

Michael TrumppLeibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Robert-Rössle-Str. 10, 13125 Berlin, Germany. broichhagen@fmp-berlin.de.ORCID http://orcid.org/0000-0002-6151-9151
Anna OliverasBerlin Institute of Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine, Hannoversche Str. 28, 10115 Berlin, Germany. Melissa.Birol@mdc-berlin.de.
Hannes GonschiorLeibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Robert-Rössle-Str. 10, 13125 Berlin, Germany. broichhagen@fmp-berlin.de.
Julia AstInstitute of Metabolism and Systems Research (IMSR), and Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham, Birmingham, UK.ORCID http://orcid.org/0000-0002-0039-4762
David J HodsonInstitute of Metabolism and Systems Research (IMSR), and Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham, Birmingham, UK.ORCID http://orcid.org/0000-0002-8641-8568
Petra KnausFreie Universität Berlin, Institute of Chemistry and Biochemistry - Biochemistry, Thielallee 63, 14195 Berlin, Germany.ORCID http://orcid.org/0000-0003-2492-1667
Martin LehmannLeibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Robert-Rössle-Str. 10, 13125 Berlin, Germany. broichhagen@fmp-berlin.de.
Melissa BirolBerlin Institute of Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine, Hannoversche Str. 28, 10115 Berlin, Germany. Melissa.Birol@mdc-berlin.de.
Johannes BroichhagenLeibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Robert-Rössle-Str. 10, 13125 Berlin, Germany. broichhagen@fmp-berlin.de.ORCID http://orcid.org/0000-0003-3084-6595
Leibniz-Forschungsinstitut für Molekulare Pharmakologie · DEMax Delbrück Center · DEChurchill Hospital · GBFreie Universität Berlin · DEUniversity of Birmingham · GB

Funding

Medical Research Council MR/N00275X/1Medical Research Council MR/S025618/1
6 · The paper itself

Abstract

Herein, we evaluate near-infrared ATTO700 as an acceptor in SNAP- and Halo-tag protein labelling for Förster Resonance Energy Transfer (FRET) by ensemble and single molecule measurements. Microscopy of cell surface proteins in live cells is perfomed including super-resolution stimulated emission by depletion (STED) nanoscopy.

Indexed as

MicroscopyNanotechnologyFluorescence Resonance Energy TransferProteinsProteins

Identifiers

PMID36427021
PMCPMC9745879
OpenAlexW4310464829

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.