ArticleAnalytical chemistry2022
Unified-Hydrophilic-Interaction/Anion-Exchange Liquid Chromatography Mass Spectrometry (Unified-HILIC/AEX/MS): A Single-Run Method for Comprehensive and Simultaneous Analysis of Polar Metabolome.
Article in Analytical chemistry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 30 citations in OpenAlex.
- High-throughput monitoring of in vitro enzymatic reactions using probe electrospray ionization-mass spectrometry.Analytical and bioanalytical chemistry · 2026Article
- Sub-3 μm porous graphitic carbon for highly polar metabolomics: orthogonal selectivity and comparison with HILIC and reversed-phase chromatography.Analytical and bioanalytical chemistry · 2026Article
- Low-Temperature HILIC Provides Enhanced Separations and Stability for LC-MS-Based Metabolomics.Journal of proteome research · 2026Article
- Preparation and application of a novel dendrimer modified silica reversed phase/hydrophilic interaction mixed-mode stationary phase.Mikrochimica acta · 2026Article
- The Role of Ionic Liquids at the Biological Interfaces in Bioelectronics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Receptor-Mitochondria Crosstalk in the Kynurenine Metabolic Pathway: Integrating Metabolomics and Clinical Mass Spectrometry.Antioxidants (Basel, Switzerland) · 2026Review
- RPLC- and HILIC-based non-targeted metabolomics workflow for blood microsamples.Metabolomics : Official journal of the Metabolomic Society · 2026Article
- Evaluation of LC/MS Methods for Hydrophilic Metabolites to Enable Integration of Human Blood Metabolome Data.Mass spectrometry (Tokyo, Japan) · 2026Article
- Untargeted analysis of hydrophilic metabolites using enhanced LC-MS separation with a pentafluoro phenylpropyl-functionalized column and prediction-based MS/MS spectrum annotation.Metabolomics : Official journal of the Metabolomic Society · 2025Article
- Environmental Applications of Mass Spectrometry for Emerging Contaminants.Molecules (Basel, Switzerland) · 2025Review
- HILIC-MS/MS Multi-targeted Method for Metabolomics Applications.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Wide-Targeted Semi-Quantitative Analysis of Acidic Glycosphingolipids in Cell Lines and Urine to Develop Potential Screening Biomarkers for Renal Cell Carcinoma.International journal of molecular sciences · 2024Article
- A Fast and Simple Solid Phase Extraction-Based Method for Glucosinolate Determination: An Alternative to the ISO-9167 Method.Foods (Basel, Switzerland) · 2024Article
- Comparison of Amine-Modified Polymeric Stationary Phases for Polar Metabolomic Analysis Based on Unified-Hydrophilic Interaction/Anion Exchange Liquid Chromatography/High-Resolution Mass Spectrometry (Unified-HILIC/AEX/HRMS).Mass spectrometry (Tokyo, Japan) · 2024Article
- Determination of Choline-Containing Compounds in Rice Bran Fermented withMass spectrometry (Tokyo, Japan) · 2024Article
- Wide-scope targeted analysis of bioactive lipids in human plasma by LC/MS/MS.Journal of lipid research · 2024Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
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Abstract
One of the technical challenges in the field of metabolomics is the development of a single-run method to detect the full complement of polar metabolites in biological samples. However, an ideal method to meet this demand has not yet been developed. Herein, we proposed a simple methodology that enables the comprehensive and simultaneous analysis of polar metabolites using unified-hydrophilic-interaction/anion-exchange liquid chromatography mass spectrometry (unified-HILIC/AEX/MS) with a polymer-based mixed amines column composed of methacrylate-based polymer particles with primary, secondary, tertiary, and quaternary amines as functional groups. The optimized unified-HILIC/AEX/MS method is composed of two consecutive chromatographic separations, HILIC-dominant separation for cationic, uncharged, and zwitterionic polar metabolites [retention times (RTs) = 0-12.8 min] and AEX-dominant separation for polar anionic metabolites (RTs = 12.8-26.5 min), by varying the ratio of acetonitrile to 40 mM ammonium bicarbonate solution (pH 9.8). A total of 400 polar metabolites were analyzed simultaneously through a combination of highly efficient separation using unified-HILIC/AEX and remarkably sensitive detection using multiple reaction monitoring-based triple quadrupole mass spectrometry (unified-HILIC/AEX/MS/MS). A nontargeted metabolomic approach using unified-HILIC/AEX high-resolution mass spectrometry (unified-HILIC/AEX/HRMS) also provided more comprehensive information on polar metabolites (3242 metabolic features) in HeLa cell extracts than the conventional HILIC/HRMS method (2068 metabolic features). Our established unified-HILIC/AEX/MS/MS and unified-HILIC/AEX/HRMS methods have several advantages over conventional techniques, including polar metabolome coverage, throughput, and accurate quantitative performance, and represent potentially useful tools for in-depth studies on metabolism and biomarker discovery.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.