ArticleIranian journal of veterinary research2022
Comparative evaluation of various diagnostic techniques for detection of
Article in Iranian journal of veterinary research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed, 5 citations in OpenAlex.
- Seroprevalence and risk factors associated with calves' cryptosporidiosis in Egypt.BMC veterinary research · 2026Article
- Molecular analysis of cryptosporidiosis on cattle farms in Gran Canaria, Canary Islands (Spain).International journal of veterinary science and medicine · 2025Article
- Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
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Abstract
Background: Aims: The current study was conducted with the objective to evaluate various diagnostic techniques (acid fast staining, negative staining, fluorescent, ELISA, PCR, nested PCR, and qPCR) for the detection of Methods: Two hundred diarrheic faecal samples from bovine calves were collected and subjected to these techniques for Results: Out of 200 faecal samples evaluated, 24% (48/200) were detected positive for Cryptosporidium using a combination of two techniques as gold standard criteria. Cohen's kappa value indicated moderate to almost perfect agreement (0.616 to 0.986) among all the techniques used in the present study. Leishman staining showed the lowest sensitivity (54.17%), while nested PCR and qPCR showed the highest sensitivity (97.92%). Diagnostic specificity of all these tests ranged from 98.68 to 100%. Conclusion: Auramine stain was used for the first time in the bovine calves in India for the detection and diagnostic comparison of
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