ReviewBiophysical reports2021
Separation of spectrally overlapping fluorophores using intra-exposure excitation modulation.
Review in Biophysical reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed.
- All-optical strategies to minimize photobleaching in reversibly switchable fluorescent proteins.Nature communications · 2025Article
- Periodic Light Modulations for Low-Cost Wide-Field Imaging of Luminescence Kinetics Under Ambient Light.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Article
- Electrochemical fluorescence modulation enables simultaneous multicolour imaging.Nature photonics · 2025Article
- Article
- Multicolor lifetime imaging and its application to HIV-1 uptake.Nature communications · 2023Article
- Latest trends in bioimaging and building a proactive network of early-career young scientists around bioimaging in Europe.Biology open · 2022Article
- Resonances at Fundamental and Harmonic Frequencies for Selective Imaging of Sine-Wave Illuminated Reversibly Photoactivatable Labels.Chemphyschem : a European journal of chemical physics and physical chemistry · 2022Article
- Super-multiplexing excitation spectral microscopy with multiple fluorescence bands.Biomedical optics express · 2022Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Multicolor fluorescence imaging is an excellent method for the simultaneous visualization of multiple structures, although it is limited by the available spectral window. More labels can be measured by distinguishing these on properties, such as their fluorescence dynamics, but usually these dynamics must be directly resolvable by the instrument. We propose an approach to distinguish emitters over a much broader range of light-induced dynamics by combining fast modulation of the light source with the detection of the time-integrated fluorescence. We demonstrate our method by distinguishing four spectrally overlapping photochromic fluorophores within
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.