ArticleBiophysical reports2021
Multimodal single-molecule microscopy with continuously controlled spectral resolution.
Article in Biophysical reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- A Dispersive Image Scanning Microscope for Multi-color High-resolution Imaging.ACS photonics · 2026Article
- SM-NucSeq, Genetic and Epigenetic Sequencing of Nucleosomes with Single-Molecule Real-Time Sequencing.Nano letters · 2026Article
- Compact Spectral Encoding Microscopy by Terrace Grating Optics.ACS photonics · 2026Article
- DeepQR: single-molecule QR codes for optical gene-expression analysis.Nanophotonics (Berlin, Germany) · 2025Article
- Hyperspectral Imaging for High Throughput Optical Spectroscopy of pL Droplets.Analytical chemistry · 2025Article
- Investigating the photophysical properties of rhodamines using a spectroscopic single-molecule fluorescence method.RSC advances · 2024Article
- Machine-Learning-Based Single-Molecule Quantification of Circulating MicroRNA Mixtures.ACS sensors · 2023Article
- Spatial mapping of the DNA adducts in cancer.DNA repair · 2023Review
- Article
- Spectroscopic single-molecule localization microscopy: applications and prospective.Nano convergence · 2023Review
- Enabling Spectrally Resolved Single-Molecule Localization Microscopy at High Emitter Densities.Nano letters · 2022Article
- Multipair Förster Resonance Energy Transfer via Spectrally Resolved Single-Molecule Detection.The journal of physical chemistry. B · 2022Article
- Live-cell fluorescence spectral imaging as a data science challenge.Biophysical reviews · 2022Review
- Can DyeCycling break the photobleaching limit in single-molecule FRET?Nano research · 2022Article
- Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Color is a fundamental contrast mechanism in fluorescence microscopy, providing the basis for numerous imaging and spectroscopy techniques. Building on spectral imaging schemes that encode color into a fixed spatial intensity distribution, here, we introduce continuously controlled spectral-resolution (CoCoS) microscopy, which allows the spectral resolution of the system to be adjusted in real-time. By optimizing the spectral resolution for each experiment, we achieve maximal sensitivity and throughput, allowing for single-frame acquisition of multiple color channels with single-molecule sensitivity and 140-fold larger fields of view compared with previous super-resolution spectral imaging techniques. Here, we demonstrate the utility of CoCoS in three experimental formats, single-molecule spectroscopy, single-molecule Förster resonance energy transfer, and multicolor single-particle tracking in live neurons, using a range of samples and 12 distinct fluorescent markers. A simple add-on allows CoCoS to be integrated into existing fluorescence microscopes, rendering spectral imaging accessible to the wider scientific community.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.