ArticleBiophysical reports2022
A common framework for single-molecule localization using sequential structured illumination.
Article in Biophysical reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 3 citations in OpenAlex.
- Optimizing Effective Labeling Efficiency in MINFLUX 3D DNA-PAINT Microscopy by Maximizing Marker Detection Probability.ACS photonics · 2026Article
- Evaluating MINFLUX experimental performance in silico.Nature communications · 2025Article
- Open-source sub-nanometer stabilization system for super-resolution fluorescence microscopy.Light, science & applications · 2025Article
- Localization of single molecules with structured illumination and structured detection.Light, science & applications · 2025Article
- MINFLUX nanoscopy enhanced with high-order vortex beams.Light, science & applications · 2025Article
- MINFLUX achieves molecular resolution with minimal photons.Nature photonics · 2025Article
- Revealing mitochondrial architecture and functions with single molecule localization microscopy.Biology of the cell · 2025Review
- Reaching the precision limit with tensor-based wavefront shaping.Nature communications · 2024Article
- 4D Single-particle tracking with asynchronous read-out single-photon avalanche diode array detector.Nature communications · 2024Article
- Simple and robust 3D MINFLUX excitation with a variable phase plate.Light, science & applications · 2024Article
- Quantifying the minimum localization uncertainty of image scanning localization microscopy.Biophysical reports · 2024Article
- Article
- Extensive Bioinformatics Analyses Reveal a Phylogenetically Conserved Winged Helix (WH) Domain (Zτ) of Topoisomerase IIα, Elucidating Its Very High Affinity for Left-Handed Z-DNA and Suggesting Novel Putative Functions.International journal of molecular sciences · 2023Article
- Real-time single-molecule 3D tracking in E. coli based on cross-entropy minimization.Nature communications · 2023Article
- Raster-scanning Donut simplifies MINFLUX and provides alternative implement on other scanning-based microscopes.Light, science & applications · 2022Article
- An alternative to MINFLUX that enables nanometer resolution in a confocal microscope.Light, science & applications · 2022Article
- Prior information improves resolution.Biophysical journal · 2022Article
- Precision in iterative modulation enhanced single-molecule localization microscopy.Biophysical journal · 2022Article
- Multiphoton single-molecule localization by sequential excitation with light minima.Light, science & applications · 2022Article
- Fluorescence nanoscopy at the sub-10 nm scale.Biophysical reviews · 2021Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Localization of single fluorescent molecules is key for physicochemical and biophysical measurements, such as single-molecule tracking and super-resolution imaging by single-molecule localization microscopy. Over the last two decades, several methods have been developed in which the position of a single emitter is interrogated with a sequence of spatially modulated patterns of light. Among them, the recent MINFLUX technique outstands for achieving a ∼10-fold improvement compared with wide-field camera-based single-molecule localization, reaching ∼1-2 nm localization precision at moderate photon counts. Here, we present a common framework for this type of measurement. Using the Cramér-Rao bound as a limit for the achievable localization precision, we benchmark reported methods, including recent developments, such as MINFLUX and MINSTED, and long-established methods, such as orbital tracking. In addition, we characterize two new proposed schemes, orbital tracking and raster scanning, with a minimum of intensity. Overall, we found that approaches using an intensity minimum have a similar performance in the central region of the excitation pattern, independent of the geometry of the excitation pattern, and that they outperform methods featuring an intensity maximum.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.