ArticlePlant physiology2023
Fast X-ray fluorescence microscopy provides high-throughput phenotyping of element distribution in seeds.
Article in Plant physiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 13 citations in OpenAlex.
- Rapid acquisition of ionomic and morphological data from plant seeds through fast X-ray fluorescence microscopy and computer vision.Plant phenomics (Washington, D.C.) · 2025Article
- The aleurone layer of cereal grains: Development, genetic regulation, and breeding applications.Plant communications · 2025Review
- Applications of synchrotron light in seed research: an array of x-ray and infrared imaging methodologies.Frontiers in plant science · 2024Article
- Grain scans: fast X-ray fluorescence microscopy for high-throughput elemental mapping of rice seeds.Plant physiology · 2023Article
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Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The concentration, chemical speciation, and spatial distribution of essential and toxic mineral elements in cereal seeds have important implications for human health. To identify genes responsible for element uptake, translocation, and storage, high-throughput phenotyping methods are needed to visualize element distribution and concentration in seeds. Here, we used X-ray fluorescence microscopy (μ-XRF) as a method for rapid and high-throughput phenotyping of seed libraries and developed an ImageJ-based pipeline to analyze the spatial distribution of elements. Using this method, we nondestructively scanned 4,190 ethyl methanesulfonate (EMS)-mutagenized M1 rice (Oryza sativa) seeds and 533 diverse rice accessions in a genome-wide association study (GWAS) panel to simultaneously measure concentrations and spatial distribution of elements in the embryo, endosperm, and aleurone layer. A total of 692 putative mutants and 65 loci associated with the spatial distribution of elements in rice seed were identified. This powerful method provides a basis for investigating the genetics and molecular mechanisms controlling the accumulation and spatial variations of mineral elements in plant seeds.
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