Evidence map›Paper›PMID 36423189›Full record

ArticleViruses2022

Evaluation of Rapid Dot-Immunoassay for Detection Orthopoxviruses Using Laboratory-Grown Viruses and Animal's Clinical Specimens.

Nikita Ushkalenko, Anna Ersh, Alexander Sergeev, Pavel Filatov, Alexander Poltavchenko

Open access · goldAbstract read
In one paragraph

Article in Viruses, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Nikita UshkalenkoState Research Center of Virology and Biotechnology "Vector", Novosibirsk Region, Koltsovo 630559, Russia.
Anna ErshState Research Center of Virology and Biotechnology "Vector", Novosibirsk Region, Koltsovo 630559, Russia.
Alexander SergeevState Research Center of Virology and Biotechnology "Vector", Novosibirsk Region, Koltsovo 630559, Russia.ORCID 0000-0001-8355-5551
Pavel FilatovState Research Center of Virology and Biotechnology "Vector", Novosibirsk Region, Koltsovo 630559, Russia.ORCID 0000-0001-7763-3808
Alexander PoltavchenkoState Research Center of Virology and Biotechnology "Vector", Novosibirsk Region, Koltsovo 630559, Russia.
State Research Center of Virology and Biotechnology VECTOR · RU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The aim of the work was an experimental evaluation of the characteristics of the kit for the rapid immunochemical detection of orthopoxviruses (OPV). The kit is based on the method of one-stage dot-immunoassay on flat protein arrays using gold conjugates and a silver developer. Rabbit polyclonal antibodies against the vaccinia virus were used as capture and detection reagents. The sensitivity of detection of OPV and the specificity of the analysis were assessed using culture crude preparations (monkeypox virus, vaccinia virus, rabbitpox virus, cowpox virus, and ectromelia virus), a suspension from a crust from a human vaccination site as well as blood and tissue suspensions of infected rabbits. It has been shown that the assay using the kit makes it possible to detect OPV within 36 min at a temperature of 18-40 °C in unpurified culture samples of the virus and clinical samples in the range of 10

Indexed as

OrthopoxvirusAnimalsHumansImmunoassayLaboratoriesMonkeypox virusRabbitsVaccinia virusclinical samplescowpox virusdetectiondot-immunoassayectromelia virusmonkeypox virusorthopoxvirusesrabbitpox virusvaccinia virus

Identifiers

PMID36423189
PMCPMC9697496
OpenAlexW4309720744

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.