Evidence map›Paper›PMID 36423135›Full record

ArticleViruses2022

Rapid and Visual Detection of Type 2 Porcine Reproductive and Respiratory Syndrome Virus by Real-Time Fluorescence-Based Reverse Transcription Recombinase-Aided Amplification.

Wenlong Xia, Yao Chen, Xue Ding, Xiaoming Liu, Huipeng Lu, Changming Guo, Hua Zhang, Zhijun Wu, Jing Huang, Zhongjun Fan and 4 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Viruses, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.1field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Review
  6. Rapid Visual Detection ofTransboundary and emerging diseases · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 3 institutions in 1 country.

Wenlong XiaJiangsu Key Laboratory for Bioresources of Saline Soils, School of Ocean and Biological Engineering, Yancheng Teachers University, Yancheng 224007, China.ORCID 0000-0002-1393-4860
Yao ChenJiangsu Key Laboratory for Bioresources of Saline Soils, School of Ocean and Biological Engineering, Yancheng Teachers University, Yancheng 224007, China.
Xue DingJiangsu Key Laboratory for Bioresources of Saline Soils, School of Ocean and Biological Engineering, Yancheng Teachers University, Yancheng 224007, China.
Xiaoming LiuCollege of Veterinary Medicine, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China.
Huipeng LuJiangsu Key Laboratory for High-Tech Research and Development of Veterinary Biopharmaceuticals, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 225300, China.
Changming GuoJiangsu Key Laboratory for High-Tech Research and Development of Veterinary Biopharmaceuticals, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 225300, China.
Hua ZhangSchool of Pharmacy, Yancheng Teachers University, Yancheng 224007, China.
Zhijun WuSchool of Pharmacy, Yancheng Teachers University, Yancheng 224007, China.
Jing HuangJiangsu Key Laboratory for Bioresources of Saline Soils, School of Ocean and Biological Engineering, Yancheng Teachers University, Yancheng 224007, China.
Zhongjun FanJiangsu Key Laboratory for Bioresources of Saline Soils, School of Ocean and Biological Engineering, Yancheng Teachers University, Yancheng 224007, China.
Shupei YuYancheng Animal Husbandry and Veterinary Station, Yancheng 224001, China.
Huaichang SunCollege of Veterinary Medicine, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China.
Shanyuan ZhuJiangsu Key Laboratory for High-Tech Research and Development of Veterinary Biopharmaceuticals, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 225300, China.
Zhi WuJiangsu Key Laboratory for High-Tech Research and Development of Veterinary Biopharmaceuticals, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 225300, China.
Jiangsu Agri-animal Husbandry Vocational College · CNYancheng Teachers University · CNYangzhou University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Porcine reproductive and respiratory syndrome (PRRS) is one of the most important diseases that has brought significant economic losses to the swine industry worldwide. Rapid and accurate PRRS virus (PRRSV) detection is one of the key factors for PRRS prevention and control. This study developed a real-time fluorescence-based reverse transcription recombinase-aided amplification (RF-RT-RAA) method for type 2 PRRSV (PRRSV-2) detection. The RF-RT-RAA assay could be performed at 42 °C for 20 min with the optimal primers and a probe. RF-RT-RAA results could be monitored using real-time fluorescence read-out or visually observed with the naked eye using a portable blue light transilluminator. The method had a strong specificity; no cross-reaction was identified with the detected common swine viruses. Moreover, the technique yielded high sensitivity with the lowest detection limit of 10

Indexed as

Porcine Reproductive and Respiratory SyndromePorcine respiratory and reproductive syndrome virusAnimalsHydrolasesRecombinasesReverse TranscriptionSwineHydrolasesRecombinasesporcine reproductive and respiratory syndrome virusrapid detectionreal-time fluorescence-based reverse transcription recombinase-aided amplification

Identifiers

PMID36423135
PMCPMC9699348
OpenAlexW4309315294

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.