Evidence map›Paper›PMID 36421176›Full record

ArticleBiosensors2022

Development of Ic-ELISA and Colloidal Gold Lateral Flow Immunoassay for the Determination of Cypermethrin in Agricultural Samples.

Lianrun Huang, Fuxuan Zhang, Fangxuan Li, Yin Jia, Minghua Wang, Xiude Hua, Limin Wang

Open access · goldAbstract read
In one paragraph

Article in Biosensors, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.9field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 14 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Lianrun HuangCollege of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Fuxuan ZhangCollege of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Fangxuan LiCollege of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Yin JiaCollege of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Minghua WangCollege of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Xiude HuaCollege of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.ORCID 0000-0003-0557-1892
Limin WangCollege of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Nanjing Agricultural University · CN

Funding

National Natural Science Foundation of China 31772194National Natural Science Foundation of China 31972311
6 · The paper itself

Abstract

Cypermethrin (CYP) is an insecticide in the pyrethroid family and is used widely in agriculture and for public health purposes. However, CYP has been shown to have negative impacts on reproduction, immunity and nerves in mammals. In this study, a monoclonal antibody (mAb) against CYP was prepared and used to establish an indirect competitive immunosorbent assay (ic-ELISA) and colloidal gold lateral flow immunoassay (LFIA) for the quantitative and qualitative determination of CYP residues in agricultural products. The half inhibition concentration of the ic-ELISA was 2.49 ng/mL, and the cut-off value and visual limit of detection of the LFIA were 0.6 and 0.3 μg/mL, respectively. The recovery rates of the ic-ELISA ranged from 78.8% to 87.6% in tomato, cabbage and romaine lettuce. The qualitative results of LFIA and quantitative results of ic-ELISA and HPLC were in good agreement in blind samples. Overall, the established ic-ELISA and LFIA proved to be accurate and rapid methods for the determination of CYP in agricultural products.

Indexed as

Gold ColloidPyrethrinsAgricultureAnimalsEnzyme-Linked Immunosorbent AssayImmunoassayMammalscypermethrinGold ColloidPyrethrinscolloidal goldcypermethrinlateral flow immunoassaymonoclonal antibody

Identifiers

PMID36421176
PMCPMC9688530
OpenAlexW4309705210

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.