Evidence map›Paper›PMID 36421008›Full record

ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2022

Studying the trafficking of labeled trodusquemine and its application as nerve marker for light-sheet and expansion microscopy.

Claudia Capitini, Luca Pesce, Giulia Fani, Giacomo Mazzamuto, Massimo Genovese, Alessandra Franceschini, Paolo Paoli, Giuseppe Pieraccini, Michael Zasloff, Fabrizio Chiti and 2 more

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In one paragraph

Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.8field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Review
  5. Studying the trafficking of labeled trodusquemine and its application as nerve marker for light-sheet and expansion microscopy.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 2 countries.

Claudia CapitiniEuropean Laboratory for Non-Linear Spectroscopy (LENS), University of Florence, Sesto Fiorentino, Italy.
Luca PesceEuropean Laboratory for Non-Linear Spectroscopy (LENS), University of Florence, Sesto Fiorentino, Italy.
Giulia FaniDepartment of Experimental and Clinical Biomedical Sciences, Section of Biochemistry, University of Florence, Florence, Italy.
Giacomo MazzamutoEuropean Laboratory for Non-Linear Spectroscopy (LENS), University of Florence, Sesto Fiorentino, Italy.
Massimo GenoveseDepartment of Experimental and Clinical Biomedical Sciences, Section of Biochemistry, University of Florence, Florence, Italy.
Alessandra FranceschiniEuropean Laboratory for Non-Linear Spectroscopy (LENS), University of Florence, Sesto Fiorentino, Italy.
Paolo PaoliDepartment of Experimental and Clinical Biomedical Sciences, Section of Biochemistry, University of Florence, Florence, Italy.
Giuseppe PieracciniCISM - Mass Spectrometry Centre, University of Florence, Sesto Fiorentino, Italy.
Michael ZasloffEnterin Inc., Philadelphia, Pennsylvania, USA.
Fabrizio ChitiDepartment of Experimental and Clinical Biomedical Sciences, Section of Biochemistry, University of Florence, Florence, Italy.
Francesco S PavoneEuropean Laboratory for Non-Linear Spectroscopy (LENS), University of Florence, Sesto Fiorentino, Italy.
Martino CalamaiEuropean Laboratory for Non-Linear Spectroscopy (LENS), University of Florence, Sesto Fiorentino, Italy.ORCID 0000-0002-4031-7235
University of Florence · ITNational Research Council · ITGeorgetown University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Trodusquemine is an aminosterol with a variety of biological and pharmacological functions, such as acting as an antimicrobial, stimulating body weight loss and interfering with the toxicity of proteins involved in the development of Alzheimer's and Parkinson's diseases. The mechanisms of interaction of aminosterols with cells are, however, still largely uncharacterized. Here, by using fluorescently labeled trodusquemine (TRO-A594 and TRO-ATTO565), we show that trodusquemine binds initially to the plasma membrane of living cells, that the binding affinity is dependent on cholesterol, and that trodusquemine is then internalized and mainly targeted to lysosomes after internalization. We also found that TRO-A594 is able to strongly and selectively bind to myelinated fibers in fixed mouse brain slices, and that it is a marker compatible with tissue clearing and light-sheet fluorescence microscopy or expansion microscopy. In conclusion, this work contributes to further characterize the biology of aminosterols and provides a new tool for nerve labeling suitable for the most advanced microscopy techniques.

Indexed as

CholestanesAnimalsCholesterolMiceMicroscopy, FluorescenceSpermine3-N-1(spermine)-7, 24-dihydroxy-5-cholestane 24-sulfateCholestanesCholesterolSpermineintracellular traffickinglipid membranenerve fiber stainingneurodegenerationoptical imagingsqualamine

Identifiers

PMID36421008
PMCPMC9827910
OpenAlexW4309896420

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.