ArticleMolecular microbiology2023
Spatial and temporal localization of cell wall associated pili in Enterococcus faecalis.
Article in Molecular microbiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 16 citations in OpenAlex.
- Significant changes in gut microbiota and SCFAs among patients with newly diagnosed acute myeloid leukemia.Frontiers in microbiology · 2025Article
- Biofilm-specific determinants of enterococci pathogen.Archives of microbiology · 2024Review
- The HtrA chaperone monitors sortase-assembled pilus biogenesis in Enterococcus faecalis.PLoS genetics · 2024Article
- Molecular basis for dual functions in pilus assembly modulated by the lid of a pilus-specific sortase.The Journal of biological chemistry · 2024Article
- Green Approach for Synthesis of Silver Nanoparticles with Antimicrobial and Antioxidant Properties from Grapevine Waste Extracts.International journal of molecular sciences · 2024Article
- Structural and functional insights of sortases and their interactions with antivirulence compounds.Current research in structural biology · 2024Review
- Tracking Cell Wall-Anchored Proteins in Gram-Positive Bacteria.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Spatial and temporal localization of cell wall associated pili in Enterococcus faecalis.Molecular microbiology · 2023Article
- Enterococcus faecalis alters endo-lysosomal trafficking to replicate and persist within mammalian cells.PLoS pathogens · 2022Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 3 countries.
Funding
Abstract
Enterococcus faecalis virulence requires cell wall-associated proteins, including the sortase-assembled endocarditis and biofilm associated pilus (Ebp), important for biofilm formation in vitro and in vivo. The current paradigm for sortase-assembled pilus biogenesis in Gram-positive bacteria is that sortases attach substrates to lipid II peptidoglycan (PG) precursors, prior to their incorporation into the growing cell wall. Contrary to prevailing dogma, by following the distribution of Ebp and PG throughout the E. faecalis cell cycle, we found that cell surface Ebp do not co-localize with newly synthesized PG. Instead, surface-exposed Ebp are localized to the older cell hemisphere and excluded from sites of new PG synthesis at the septum. Moreover, Ebp deposition on the younger hemisphere of the E. faecalis diplococcus appear as foci adjacent to the nascent septum. We propose a new model whereby sortase substrate deposition can occur on older PG rather than at sites of new cell wall synthesis. Consistent with this model, we demonstrate that sequestering lipid II to block PG synthesis via ramoplanin, does not impact new Ebp deposition at the cell surface. These data support an alternative paradigm for sortase substrate deposition in E. faecalis, in which Ebp are anchored directly onto uncrosslinked cell wall, independent of new PG synthesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.