Evidence map›Paper›PMID 36420904›Full record

ArticleNucleic acids research2023

SEVA 4.0: an update of the Standard European Vector Architecture database for advanced analysis and programming of bacterial phenotypes.

Esteban Martínez-García, Sofía Fraile, Elena Algar, Tomás Aparicio, Elena Velázquez, Belén Calles, Huseyin Tas, Blas Blázquez, Bruno Martín, Clara Prieto and 12 more

Abstract read
In one paragraph

Article in Nucleic acids research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.

0numbers the graph read from it
0cells of the map it votes in
70citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

70 citing papers in PubMed.

  1. Metabolic Engineering ofMicroorganisms · 2026
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  6. A periplasmic metallochaperone (PmcY) couples ZnAntimicrobial agents and chemotherapy · 2026
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  7. Multi-gene Co-expression systems inSynthetic and systems biotechnology · 2026
    Review
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  10. Review
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  13. Pseudopaline-mediated zinc uptake byInfection and immunity · 2026
    Article
  14. Article
  15. Article
  16. Article
  17. Review
  18. A biosensor toolbox for monitoring recombinant protein production inFrontiers in bioengineering and biotechnology · 2026
    Article
  19. Manganese homeostasis supportsCurrent research in microbial sciences · 2026
    Article
  20. Article

10 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

22 authors.

Esteban Martínez-GarcíaSystems Biology Department, Centro Nacional de Biotecnología (CNB-CSIC), 28049 Cantoblanco-Madrid, Spain.ORCID 0000-0001-8475-7286
Sofía FraileSystems Biology Department, Centro Nacional de Biotecnología (CNB-CSIC), 28049 Cantoblanco-Madrid, Spain.ORCID 0000-0002-6476-7513
Elena AlgarSystems Biology Department, Centro Nacional de Biotecnología (CNB-CSIC), 28049 Cantoblanco-Madrid, Spain.ORCID 0000-0002-0178-3153
Tomás AparicioSystems Biology Department, Centro Nacional de Biotecnología (CNB-CSIC), 28049 Cantoblanco-Madrid, Spain.ORCID 0000-0002-0133-0461
Elena VelázquezSystems Biology Department, Centro Nacional de Biotecnología (CNB-CSIC), 28049 Cantoblanco-Madrid, Spain.ORCID 0000-0001-6648-1634
Belén CallesSystems Biology Department, Centro Nacional de Biotecnología (CNB-CSIC), 28049 Cantoblanco-Madrid, Spain.ORCID 0000-0003-0603-0115
Huseyin TasSystems Biology Department, Centro Nacional de Biotecnología (CNB-CSIC), 28049 Cantoblanco-Madrid, Spain.ORCID 0000-0003-4804-2237
Blas BlázquezSystems Biology Department, Centro Nacional de Biotecnología (CNB-CSIC), 28049 Cantoblanco-Madrid, Spain.ORCID 0000-0003-4099-6909
Bruno MartínScienseed SL, 28020 Madrid,Spain.
Clara PrietoScienseed SL, 28020 Madrid,Spain.
Lucas Sánchez-SampedroScienseed SL, 28020 Madrid,Spain.
Morten H H NørholmThe Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, 2800 Kongens Lyngby, Denmark.ORCID 0000-0002-7871-5191
Daniel C VolkeThe Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, 2800 Kongens Lyngby, Denmark.
Nicolas T WirthThe Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, 2800 Kongens Lyngby, Denmark.
Pavel DvořákDepartment of Experimental Biology, Faculty of Science, Masaryk University, Brno 62500 Czech Republic.
Lorea AlejaldreCentro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (INIA-CSIC), Pozuelo de Alarcón 28223, Spain.ORCID 0000-0003-3086-5446
Lewis GrozingerCentro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (INIA-CSIC), Pozuelo de Alarcón 28223, Spain.
Matthew CrowtherCentro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (INIA-CSIC), Pozuelo de Alarcón 28223, Spain.
Angel Goñi-MorenoCentro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (INIA-CSIC), Pozuelo de Alarcón 28223, Spain.ORCID 0000-0002-2097-2507
Pablo I NikelThe Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, 2800 Kongens Lyngby, Denmark.ORCID 0000-0002-9313-7481
Juan NogalesSystems Biology Department, Centro Nacional de Biotecnología (CNB-CSIC), 28049 Cantoblanco-Madrid, Spain.ORCID 0000-0002-4961-0833
Víctor de LorenzoSystems Biology Department, Centro Nacional de Biotecnología (CNB-CSIC), 28049 Cantoblanco-Madrid, Spain.ORCID 0000-0002-6041-2731

Funding

European Research Council 101044360
6 · The paper itself

Abstract

The SEVA platform (https://seva-plasmids.com) was launched one decade ago, both as a database (DB) and as a physical repository of plasmid vectors for genetic analysis and engineering of Gram-negative bacteria with a structure and nomenclature that follows a strict, fixed architecture of functional DNA segments. While the current update keeps the basic features of earlier versions, the platform has been upgraded not only with many more ready-to-use plasmids but also with features that expand the range of target species, harmonize DNA assembly methods and enable new applications. In particular, SEVA 4.0 includes (i) a sub-collection of plasmids for easing the composition of multiple DNA segments with MoClo/Golden Gate technology, (ii) vectors for Gram-positive bacteria and yeast and [iii] off-the-shelf constructs with built-in functionalities. A growing collection of plasmids that capture part of the standard-but not its entirety-has been compiled also into the DB and repository as a separate corpus (SEVAsib) because of its value as a resource for constructing and deploying phenotypes of interest. Maintenance and curation of the DB were accompanied by dedicated diffusion and communication channels that make the SEVA platform a popular resource for genetic analyses, genome editing and bioengineering of a large number of microorganisms.

Indexed as

BacteriaDatabases, FactualCloning, MolecularDNAGenetic VectorsPhenotypePlasmidsDNA

Identifiers

PMID36420904
PMCPMC9825617

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.