Evidence map›Paper›PMID 36420055›Full record

ArticleMaterials today. Bio2022

Recombinant protein condensation inside

Bartosz Gabryelczyk, Fred-Eric Sammalisto, Julie-Anne Gandier, Jianhui Feng, Grégory Beaune, Jaakko V I Timonen, Markus B Linder

Abstract read
In one paragraph

Article in Materials today. Bio, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Liquid-liquid phase separation enables chromatography-free purification and high-performance spidroin-amyloid hybrid silk fibers.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  2. Article
  3. Engineered membraneless organelles inSynthetic and systems biotechnology · 2025
    Article
  4. Review
  5. Review
  6. Article
  7. Article
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Bartosz GabryelczykDepartment of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University, Espoo, Finland.
Fred-Eric SammalistoDepartment of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University, Espoo, Finland.
Julie-Anne GandierDepartment of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University, Espoo, Finland.
Jianhui FengDepartment of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University, Espoo, Finland.
Grégory BeauneDepartment of Applied Physics, School of Science, Aalto University, Espoo, Finland.
Jaakko V I TimonenDepartment of Applied Physics, School of Science, Aalto University, Espoo, Finland.
Markus B LinderDepartment of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University, Espoo, Finland.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Recombinant expression of proteins destined to form biological materials often results in poor production yields or loss of their function due to premature aggregation. Recently, liquid-liquid phase separation has been proposed as a mechanism to control protein solubility during expression and accumulation in the cytoplasm. Here, we investigate this process

Indexed as

Intracellular condensateLiquid-liquid phase separationProtein-based materialProtein condensationProtein expression in E. coliSpider silk

Identifiers

PMID36420055
PMCPMC9677211

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.