ArticlePlant communications2023
The PtoTCP20-miR396d-PtoGRF15 module regulates secondary vascular development in Populus.
Article in Plant communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 20 citations in OpenAlex.
- The MdERF1B-miR482c-Horticulture research · 2026Article
- A MYB-Related Transcriptional Module Cooperatively Represses Xylem Development in Poplar.Plant biotechnology journal · 2026Article
- The multifaceted regulatory network of microRNA396: Orchestrating plant plasticity through coordinating development and various stresses.Journal of integrative plant biology · 2026Review
- MicroRNA-Mediated Post-transcriptional Regulation of Wood Property Traits in Eucalyptus tereticornis.Biochemical genetics · 2026Article
- 'Candidatus Liberibacter Asiaticus' Effector SECP8 Subverts Salicylic Acid-Mediated Citrus Immunity via a Two-Pronged Impairment of CsTCP15 Dimerization.Plant biotechnology journal · 2026Article
- PagMIR166c targets PagECH2 to regulate cambial differentiation into secondary xylem, cell expansion, and lignin deposition via different auxin signaling pathways in poplar stems.The New phytologist · 2025Article
- Silencing of miR169a improves drought stress by enhancing vascular architecture, ROS scavenging, and photosynthesis ofFrontiers in plant science · 2025Article
- Integrative Transcriptomic and Small RNA Analysis Uncovers Key Genes for Cold Resistance in Rice.Genes · 2024Article
- MicroRNA analysis reveals 2 modules that antagonistically regulate xylem tracheary element development in Arabidopsis.The Plant cell · 2024Article
- Growth-regulating factor 15-mediated vascular cambium differentiation positively regulates wood formation in hybrid poplar (Frontiers in plant science · 2024Article
- Transcriptome and miRNAs Profiles Reveal Regulatory Network and Key Regulators of Secondary Xylem Formation in "84K" Poplar.International journal of molecular sciences · 2023Article
- PlantForestry research · 2023Review
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Secondary vascular development is a key biological characteristic of woody plants and the basis of wood formation. Our understanding of gene expression regulation and dynamic changes in microRNAs (miRNAs) during secondary vascular development is still limited. Here we present an integrated analysis of the miRNA and mRNA transcriptome of six phase-specific tissues-the shoot apex, procambium, primary vascular tissue, cambium, secondary phloem, and secondary xylem-in Populus tomentosa. Several novel regulatory modules, including the PtoTCP20-miR396d-PtoGRF15 module, were identified during secondary vascular development in Populus. A series of biochemical and molecular experiments confirmed that PtoTCP20 activated transcription of the miR396d precursor gene and that miR396d targeted PtoGRF15 to downregulate its expression. Plants overexpressing miR396d (35S:miR396d) showed enhanced secondary growth and increased xylem production. Conversely, during the transition from primary to secondary vascular development, plants with downregulated PtoTCP20expression (PtoTCP20-SRDX), downregulated miR396 expression (35S:STTM396), and PtoGRF15 overexpression (35S:PtoGRF15) showed delayed secondary growth. Novel regulatory modules were identified by integrated analysis of the miRNA and mRNA transcriptome, and the regulatory role of the PtoTCP20-miR396d-PtoGRF15 signaling cascade in secondary vascular development was validated in Populus, providing information to support improvements in forest cultivation and wood properties.
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