Evidence map›Paper›PMID 36417865›Full record

ArticleCell reports2022

Single-nucleus RNA-seq reveals that MBD5, MBD6, and SILENZIO maintain silencing in the vegetative cell of developing pollen.

Lucia Ichino, Colette L Picard, Jaewon Yun, Meera Chotai, Shuya Wang, Evan K Lin, Ranjith K Papareddy, Yan Xue, Steven E Jacobsen

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
6.9field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 35 citations in OpenAlex.

  1. Review
  2. Article
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  8. Epigenetics in the modern era of crop improvements.Science China. Life sciences · 2025
    Review
  9. Article
  10. Article
  11. Roles of methyl-CpG-binding-domain (MBD) protiens in regulation of biological processes in plants.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025
    Review
  12. Article
  13. Article
  14. Article
  15. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Lucia IchinoMolecular Biology Institute, University of California Los Angeles, Los Angeles, CA 90095, USA; Department of Molecular, Cell and Developmental Biology, University of California Los Angeles, Los Angeles, CA 90095, USA.
Colette L PicardDepartment of Molecular, Cell and Developmental Biology, University of California Los Angeles, Los Angeles, CA 90095, USA.
Jaewon YunDepartment of Molecular, Cell and Developmental Biology, University of California Los Angeles, Los Angeles, CA 90095, USA.
Meera ChotaiDepartment of Molecular, Cell and Developmental Biology, University of California Los Angeles, Los Angeles, CA 90095, USA.
Shuya WangMolecular Biology Institute, University of California Los Angeles, Los Angeles, CA 90095, USA; Department of Molecular, Cell and Developmental Biology, University of California Los Angeles, Los Angeles, CA 90095, USA.
Evan K LinDepartment of Molecular, Cell and Developmental Biology, University of California Los Angeles, Los Angeles, CA 90095, USA.
Ranjith K PapareddyDepartment of Molecular, Cell and Developmental Biology, University of California Los Angeles, Los Angeles, CA 90095, USA.
Yan XueDepartment of Molecular, Cell and Developmental Biology, University of California Los Angeles, Los Angeles, CA 90095, USA.
Steven E JacobsenMolecular Biology Institute, University of California Los Angeles, Los Angeles, CA 90095, USA; Department of Molecular, Cell and Developmental Biology, University of California Los Angeles, Los Angeles, CA 90095, USA; Eli and Edyth Broad Center of Regenerative Medicine and Stem Cell Research, University of California Los Angeles, Los Angeles, CA 90095, USA; Howard Hughes Medical Institute (HHMI), UCLA, Los Angeles, CA 90095, USA. Electronic address: jacobsen@ucla.edu.
University of California, Los Angeles · US

Funding

Epigenetic gene regulation in ArabidopsisR35GM130272 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI JACOBSEN, STEVEN E · 2019 to 2023
$1.6M
Characterization of the function of gene body DNA methylationF32GM136115 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI PICARD, COLETTE LAFONTAINE · 2020 to 2022
$201k
NIGMS NIH HHS F32 GM136115NIGMS NIH HHS R35 GM130272
6 · The paper itself

Abstract

Silencing of transposable elements (TEs) drives the evolution of numerous redundant mechanisms of transcriptional regulation. Arabidopsis MBD5, MBD6, and SILENZIO act as TE repressors downstream of DNA methylation. Here, we show, via single-nucleus RNA-seq of developing male gametophytes, that these repressors are critical for TE silencing in the pollen vegetative cell, a companion cell important for fertilization that undergoes chromatin decompaction. Instead, other silencing mutants (met1, ddm1, mom1, morc) show loss of silencing in all pollen nucleus types and somatic cells. We show that TEs repressed by MBD5/6 gain chromatin accessibility in wild-type vegetative nuclei despite remaining silent, suggesting that loss of DNA compaction makes them sensitive to loss of MBD5/6. Consistently, crossing mbd5/6 to histone 1 mutants, which have decondensed chromatin in leaves, reveals derepression of MBD5/6-dependent TEs in leaves. MBD5/6 and SILENZIO thus act as a silencing system particularly important when chromatin compaction is compromised.

Indexed as

ArabidopsisArabidopsis ProteinsChromatinDNA-Binding ProteinsDNA (Cytosine-5-)-MethyltransferasesDNA Transposable ElementsPollenRNA-SeqTranscription FactorsArabidopsis ProteinsChromatinDNA-Binding ProteinsDNA (Cytosine-5-)-MethyltransferasesDNA Transposable ElementsMBD6 protein, ArabidopsisMET1 protein, ArabidopsisTranscription FactorsArabidopsischromatinCP: Developmental biologyCP: PlantsDNA methylationepigeneticsmale germlineMBDmethyl readerspollensingle-cell analysistransposable elements

Identifiers

PMID36417865
PMCPMC9770095
OpenAlexW4309679147

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.