Evidence map›Paper›PMID 36417751›Full record

ArticleACS synthetic biology2022

Cell-Free Protein Synthesis of Particulate Methane Monooxygenase into Nanodiscs.

Christopher W Koo, Jasmine M Hershewe, Michael C Jewett, Amy C Rosenzweig

Open access · greenAbstract read
In one paragraph

Article in ACS synthetic biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.3field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 15 citations in OpenAlex.

  1. Review
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  8. Engineering transmembrane signal transduction in synthetic membranes using two-component systems.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Christopher W KooDepartment of Molecular Biosciences and of Chemistry, Northwestern University, Evanston, Illinois60208, United States.
Jasmine M HersheweDepartment of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois60208, United States.
Michael C JewettDepartment of Chemical and Biological Engineering, Northwestern University, Evanston, Illinois60208, United States.ORCID 0000-0003-2948-6211
Amy C RosenzweigDepartment of Molecular Biosciences and of Chemistry, Northwestern University, Evanston, Illinois60208, United States.ORCID 0000-0001-8472-4134
Northwestern University · US

Funding

Tumor Environment and Metastasis (TEAM) Research ProgramP30CA060553 · NCI · NORTHWESTERN UNIVERSITY AT CHICAGO · PI LEONIDAS C. PLATANIAS · 1993 to 2026
$153.9M
Metalloenzymes and metal homeostasisR35GM118035 · NIGMS · NORTHWESTERN UNIVERSITY · PI AMY C. ROSENZWEIG · 2016 to 2026
$7.1M
Molecular Biophysics Training Program at Northwestern UniversityT32GM008382 · NIGMS · NORTHWESTERN UNIVERSITY · PI RADHAKRISHNAN, ISHWAR · 1990 to 2020
$4.3M
NCI NIH HHS P30 CA060553NIGMS NIH HHS R35 GM118035NIGMS NIH HHS T32 GM008382
6 · The paper itself

Abstract

Particulate methane monooxygenase (pMMO) is a multi-subunit membrane metalloenzyme used by methanotrophic bacteria to convert methane to methanol. A major hurdle to studying pMMO is the lack of a recombinant expression system, precluding investigation of individual residues by mutagenesis and hampering a complete understanding of its mechanism. Here, we developed an

Indexed as

Methylococcus capsulatusCopperOxidation-ReductionOxygenasesCoppermethane monooxygenaseOxygenasescell free protein synthesiscoppermembrane proteinmethanotrophparticulate methane monooxygenase

Identifiers

PMID36417751
PMCPMC9910172
OpenAlexW4309809319

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.