ArticleAngewandte Chemie (International ed. in English)2023
Light-Activated Translation of Different mRNAs in Cells via Wavelength-Dependent Photouncaging.
Article in Angewandte Chemie (International ed. in English), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Circular Photocaged mRNA for Light-induced Late-stage Activation of Translation.Angewandte Chemie (International ed. in English) · 2026Article
- Toward Chromoselective Transformations in Biological Systems: Perspectives and Challenges.Angewandte Chemie (International ed. in English) · 2026Review
- Wash-Free Multi-Target Super-Resolution Microscopy With Photocaged DNA Labels.Angewandte Chemie (International ed. in English) · 2026Article
- Small-Molecule Activation of mRNA Translation by Click-to-Release Reaction in Cells.Angewandte Chemie (International ed. in English) · 2026Article
- The 5' Cap Epitranscriptome and Beyond: Natural and Engineered 5' Cap Modifications for Optimizing mRNA Therapeutics and Functional Studies.ChemMedChem · 2026Review
- Spatiotemporal control of translation in live zebrafish embryos via photoprotected mRNAs.Communications chemistry · 2025Article
- Photoactivatable mRNA 5' Cap Analogs for RNA-Protein Crosslinking.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Photochemical Action Plots Map Orthogonal Reactivity in Photochemical Release Systems.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Article
- Cap analogs with a hydrophobic photocleavable tag enable facile purification of fully capped mRNA with various cap structures.Nature communications · 2023Article
- Orthogonal Light-Activated DNA for Patterned Biocomputing within Synthetic Cells.Journal of the American Chemical Society · 2023Article
- Light-Activated Translation of Different mRNAs in Cells via Wavelength-Dependent Photouncaging.Angewandte Chemie (International ed. in English) · 2023Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The 5' cap is a hallmark of eukaryotic mRNA involved in the initiation of translation. Its modification with a single photo-cleavable group can bring translation of mRNA under the control of light. However, UV irradiation causes cell stress and downregulation of translation. Furthermore, complex processes often involve timed expression of more than one gene. The approach would thus greatly benefit from the ability to photo-cleave by blue light and to control more than one mRNA at a time. We report the synthesis of a 5' cap modified with a 7-(diethylamino)coumarin (CouCap) and adapted conditions for in vitro transcription. Translation of the resulting CouCap-mRNA is muted in vitro and in mammalian cells, and can be initiated by irradiation with 450 nm. The native cap is restored and no non-natural residues nor sequence alterations remain in the mRNA. Multiplexing for two different mRNAs was achieved by combining cap analogs with coumarin- and ortho-nitrobenzyl-based photo-cleavable groups.
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