ArticleThe Journal of cell biology2023
Fibroblasts secrete fibronectin under lamellipodia in a microtubule- and myosin II-dependent fashion.
Article in The Journal of cell biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 18 citations in OpenAlex.
- Mechanical forces stimulate Golgi export.The Journal of cell biology · 2026Article
- Biomimetic injectable engineered hierarchical porous microspheres for enhanced synergistic cell therapy of critical limb ischemia.Bioactive materials · 2026Article
- Acidosis Regulates Microtubule Dynamics via the β1 Integrin/RhoA/CRMP-2 Axis.Journal of the American Chemical Society · 2026Article
- Aligned Fibronectin Microenvironment Temporally Facilitates Profibrotic Fibroblast Activation via Integrin α5β1.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Cellular Snowballing: Cell Adhesion and Migration Drive the Self-Assembly of Cell-Microgel Biohybrid Spheroids.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- pH-sensitive fibronectin nanogels combined with UTMD for anti-atherosclerosis treatment through anti-inflammatory and antioxidant effects.Materials today. Bio · 2025Article
- Spatiotemporal dynamic changes of meningeal microenvironment influence meningeal lymphatic function following subarachnoid hemorrhage: from inflammatory response to tissue remodeling.Journal of neuroinflammation · 2025Article
- Fibronectin matrix assembly at a glance.Journal of cell science · 2025Review
- Talin2 binds to non-muscle myosin IIa and regulates cell attachment and fibronectin secretion.Scientific reports · 2024Article
- Tissue Morphogenesis Through Dynamic Cell and Matrix Interactions.Annual review of cell and developmental biology · 2023Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Fibronectin (FN) is an essential structural and regulatory component of the extracellular matrix (ECM), and its binding to integrin receptors supports cell adhesion, migration, and signaling. Here, using live-cell microscopy of fibroblasts expressing FN tagged with a pH-sensitive fluorophore, we show that FN is secreted predominantly at the ventral surface of cells in an integrin-independent manner. Locally secreted FN then undergoes β1 integrin-dependent fibrillogenesis. We find that the site of FN secretion is regulated by cell polarization, which occurs in bursts under stabilized lamellipodia at the leading edge. Moreover, analysis of FN secretion and focal adhesion dynamics suggest that focal adhesion formation precedes FN deposition and that deposition continues during focal adhesion disassembly. Lastly, we show that the polarized FN deposition in spreading and migrating cells requires both intact microtubules and myosin II-mediated contractility. Thus, while FN secretion does not require integrin binding, the site of exocytosis is regulated by membrane and cytoskeletal dynamics with secretion occurring after new adhesion formation.
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