ArticleFood science of animal resources2022
Optimal Pre-Plating Method of Chicken Satellite Cells for Cultured Meat Production.
Article in Food science of animal resources, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 26 citations in OpenAlex.
- MuSC cultivation for cultured meat production: recent advances, current limitations, and future directions.Food science of animal resources · 2026Review
- Animal-derived components in cultivated meat research and their alternatives.NPJ science of food · 2025Review
- Taurine effects on cell proliferation and gene expression of apoptosis and oxidative stress in broiler satellite cells under heat stress.Journal of animal science and technology · 2025Article
- Comparative Transcriptome Analysis Reveals the Role of the FST Gene in Goose Muscle Development.Animals : an open access journal from MDPI · 2025Article
- From Development to Regeneration: Insights into Flight Muscle Adaptations from Bat Muscle Cell Lines.Cells · 2025Article
- Establishment of chicken muscle and adipogenic cell cultures for cultivated meat production.Frontiers in nutrition · 2025Article
- Effect of Adding Cultured Meat Tissue on Physicochemical and Taste Characteristics of Hybrid Cultured Meat Manufactured Using Wet-Spinning.Food science of animal resources · 2024Article
- Article
- Effect of Chicken Age on Proliferation and Differentiation Abilities of Muscle Stem Cells and Nutritional Characteristics of Cultured Meat Tissue.Food science of animal resources · 2024Article
- Industrial Research and Development on the Production Process and Quality of Cultured Meat Hold Significant Value: A Review.Food science of animal resources · 2024Review
- Methods to Isolate Muscle Stem Cells for Cell-Based Cultured Meat Production: A Review.Animals : an open access journal from MDPI · 2024Review
- Current Research, Industrialization Status, and Future Perspective of Cultured Meat.Food science of animal resources · 2024Review
- Current technology and industrialization status of cell-cultivated meat.Journal of animal science and technology · 2024Review
- Study on the current research trends and future agenda in animal products: an Asian perspective.Journal of animal science and technology · 2023Review
- Effect of standard and physiological cell culture temperatures onFrontiers in physiology · 2023Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To establish a pre-plating method of chicken satellite cells with high purity, pre-plating was performed under culture conditions of 37°C and 41°C, and the pre-plating time was set from a total of 3 hours to 6 hours in consideration of the cell attachment time. The purity of the cells was confirmed by staining paired box protein 7 (Pax7) after proliferation, and Pax7 expression was the highest in culture flasks shaken for 2 hours after incubation at 41°C for 2 hours to prevent the attachment of satellite cells (p<0.05). Also, when pre-plating and proliferation were performed at 37°C and 41°C, the Pax7 expression rate was higher at 41°C. The differentiation capabilities of the three groups (T3, T6, and T7) with high Pax7 expression were compared and the fusion index (%) and myotube formation area (%) determined by myosin heavy chain (MHC) staining was calculated. The T6 and T7 groups, which were cultured at 41°C, showed significantly higher values than the T3 group (p<0.05). There was no significant difference in the expression of Pax7 and MHC between the T6 and T7 groups (p>0.05). These results suggest that pre-plating at 41°C for a total of 4 hours was the most efficient in terms of cost and time for purifying chicken satellite cells for cultured meat.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.