ArticleJournal of advanced research2023
The GhMAP3K62-GhMKK16-GhMPK32 kinase cascade regulates drought tolerance by activating GhEDT1-mediated ABA accumulation in cotton.
Article in Journal of advanced research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 45 citations in OpenAlex.
- Article
- The mitogen-activated protein kinase GhMPK3 participates in ethylene signaling regulating fiber elongation in cotton (Gossypium hirsutum).Plant cell reports · 2026Article
- The OsGSK1-OsBZR3-OsJAZ4 module integrates nitrate signaling with brassinosteroid and jasmonic acid crosstalk to drive cell wall remodeling for salt recovery in rice.Plant communications · 2026Article
- Harnessing polyploidy for climate-resilient crops: Lessons from the evolutionary model, allotetraploid cotton.Proceedings of the National Academy of Sciences of the United States of America · 2026Review
- Transcriptomic Analyses and Weighted Gene Co-Expression Network Analysis (WGCNA) Identify Key Drought-Responsive Genes in Rice Roots (Plants (Basel, Switzerland) · 2026Article
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- Cloning and Functional Analysis of thePlants (Basel, Switzerland) · 2026Article
- NtLYK5 negatively regulates drought resistance in tobacco through HPlant cell reports · 2025Article
- Integrative physiological and transcriptomic analysis provides insights on the molecular basis of ABA-enhanced drought tolerance in pear (Pyrus betulaefolia).BMC plant biology · 2025Article
- 5-Aminolevulinic Acid (5-ALA)-Induced Drought Resistance in Maize Seedling Root at Physiological and Transcriptomic Levels.International journal of molecular sciences · 2024Article
- CRISPR-Cas9-mediated construction of a cotton CDPK mutant library for identification of insect-resistance genes.Plant communications · 2024Article
- Cotton Bollworm (H. armigera) Effector PPI5 Targets FKBP17-2 to Inhibit ER Immunity and JA/SA Responses, Enhancing Insect Feeding.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Genome-Wide Identification of MKK Gene Family and Response to Hormone and Abiotic Stress in Rice.Plants (Basel, Switzerland) · 2024Article
- Comprehensive molecular evolutionary analysis of small heat shock proteins in five diploid Gossypium species.The plant genome · 2024Article
- Bioinformatic Identification and Expression Analyses of the MAPK-MAP4K Gene Family Reveal a Putative Functional MAP4K10-MAP3K7/8-MAP2K1/11-MAPK3/6 Cascade in Wheat (Plants (Basel, Switzerland) · 2024Article
- Article
- Spatiotemporal transcriptomic plasticity in barley roots: unravelling water deficit responses in distinct root zones.BMC genomics · 2024Article
- Upregulation of Wheat Heat Shock Transcription FactorInternational journal of molecular sciences · 2024Article
- Integrated PacBio SMRT and Illumina sequencing uncovers transcriptional and physiological responses to drought stress in whole-plantFrontiers in genetics · 2024Article
- Water-saving techniques: physiological responses and regulatory mechanisms of crops.Advanced biotechnology · 2023Review
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionDrought is the principal abiotic stress that severely impacts cotton (Gossypium hirsutum) growth and productivity. Upon sensing drought, plants activate stress-related signal transduction pathways, including ABA signal and mitogen-activated protein kinase (MAPK) cascade. However, as the key components with the fewest members in the MAPK cascade, the function and regulation of GhMKKs need to be elucidated. In addition, the relationship between MAPK module and the ABA core signaling pathway remains incompletely understood.
objectiveHere we aim to elucidate the molecular mechanism of cotton response to drought, with a focus on mitogen-activated protein kinase (MAPK) cascades activating ABA signaling.
methodsBiochemical, molecular and genetic analysis were used to study the GhMAP3K62-GhMKK16-GhMPK32-GhEDT1 pathway genes.
resultsA nucleus- and membrane-localized MAPK cascade pathway GhMAP3K62-GhMKK16-GhMPK32, which targets and phosphorylates the nuclear-localized transcription factor GhEDT1, to activate downstream GhNCED3 to mediate ABA-induced stomatal closure and drought response was characterized in cotton. Overexpression of GhMKK16 promotes ABA accumulation, and enhances drought tolerance via regulating stomatal closure under drought stress. Conversely, RNAi-mediated knockdown of GhMKK16 expression inhibits ABA accumulation, and reduces drought tolerance. Virus-induced gene silencing (VIGS)-mediated knockdown of either GhMAP3K62, GhMPK32 or GhEDT1 expression represses ABA accumulation and reduces drought tolerance through inhibiting stomatal closure. Expression knockdown of GhMPK32 or GhEDT1 in GhMKK16-overexpressing cotton reinstates ABA content and stomatal opening-dependent drought sensitivity to wild type levels. GhEDT1 could bind to the HD boxes in the promoter of GhNCED3 to activate its expression, resulting in ABA accumulation. We propose that the MAPK cascade GhMAP3K62-GhMKK16-GhMPK32 pathway functions on drought response through ABA-dependent stomatal movement in cotton.
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