Evidence map›Paper›PMID 36413318›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Protocols for Studying Protein Stability in an Arabidopsis Protoplast Transient Expression System.

Séverine Planchais, Laurent Camborde, Isabelle Jupin

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Séverine PlanchaisLaboratoire de Virologie Moléculaire, Institut Jacques Monod, CNRS, UMR 7592, Univ. Paris-Diderot, Sorbonne Paris Cité, Paris, France.
Laurent CambordeLaboratoire de Virologie Moléculaire, Institut Jacques Monod, CNRS, UMR 7592, Univ. Paris-Diderot, Sorbonne Paris Cité, Paris, France.
Isabelle JupinLaboratoire de Virologie Moléculaire, Institut Jacques Monod, CNRS, UMR 7592, Univ. Paris-Diderot, Sorbonne Paris Cité, Paris, France. isabelle.jupin@ijm.fr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Protein stability influences many aspects of biology, and measuring their stability in vivo can provide important insights into biological systems.This chapter describes in detail two methods to assess the stability of a specific protein based on its transient expression in Arabidopsis protoplasts. First, a pulse-chase assay based on radioactive metabolic labeling of cellular proteins, followed by immunoprecipitation of the protein of interest. The decrease in radioactive signal is monitored over time and can be used to determine the protein's half-life.Alternatively, we also present a nonradioactive assay based on the use of reporter proteins, whose ratio can be quantified. This assay can be used to determine the relative stability of a protein of interest under specific conditions.

Indexed as

ArabidopsisImmunoprecipitationProteinsProtein StabilityProtoplastsProteinsArabidopsis thalianaProtein stabilityProtoplastsPulse chaseTransient expressionUPR assay

Identifiers

PMID36413318

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.