Evidence map›Paper›PMID 36411412›Full record

ArticleBMC genomics2022

Proteomic analysis reveals that aging rabbit vocal folds are more vulnerable to changes caused by systemic dehydration.

Naila C do Nascimento, Taylor W Bailey, Andrea P Santos, Chenwei Duan, Rodrigo Mohallem, Jackeline Franco, Uma K Aryal, Jun Xie, Abigail Cox, M Preeti Sivasankar

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Naila C do NascimentoDepartment of Speech, Language, and Hearing Sciences, Purdue University, West Lafayette, Indiana, 47907, USA. ncannes@purdue.edu.
Taylor W BaileyDepartment of Comparative Pathobiology, Purdue University, West Lafayette, Indiana, 47907, USA.
Andrea P SantosDepartment of Comparative Pathobiology, Purdue University, West Lafayette, Indiana, 47907, USA.
Chenwei DuanWeldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana, 47907, USA.
Rodrigo MohallemDepartment of Comparative Pathobiology, Purdue University, West Lafayette, Indiana, 47907, USA.
Jackeline FrancoPurdue Proteomics Facility, Bindley Bioscience Center, Discovery Park, Purdue University, West Lafayette, Indiana, 47907, USA.
Uma K AryalDepartment of Comparative Pathobiology, Purdue University, West Lafayette, Indiana, 47907, USA.
Jun XieDepartment of Statistics, Purdue University, West Lafayette, Indiana, 47907, USA.
Abigail CoxDepartment of Comparative Pathobiology, Purdue University, West Lafayette, Indiana, 47907, USA.
M Preeti SivasankarDepartment of Speech, Language, and Hearing Sciences, Purdue University, West Lafayette, Indiana, 47907, USA.
Purdue University West Lafayette · US

Funding

Pathobiology and biomechanics of vocal fold dehydrationR01DC015545 · NIDCD · PURDUE UNIVERSITY · PI COX, ABIGAIL, SIVASANKAR, PREETI M. · 2016 to 2020
$1.6M
NIDCD NIH HHS R01 DC015545
6 · The paper itself

Abstract

backgroundOlder adults are more prone to develop systemic dehydration. Systemic dehydration has implications for vocal fold biology by affecting gene and protein expression. The objective of this study was to quantify vocal fold protein changes between two age groups and hydration status, and to investigate the interaction of age and hydration status on protein expression, which has not been investigated in the context of vocal folds before. Comparative proteomics was used to analyze the vocal fold proteome of 6.5-month-old and > 3-year-old rabbits subjected to water ad libitum or water volume restriction protocol.

resultsYoung and older adult rabbits (n = 22) were either euhydrated (water ad libitum) or dehydrated by water volume restriction. Dehydration was confirmed by body weight loss of - 5.4% and - 4.6% in young and older groups, respectively, and a 1.7-fold increase of kidney renin gene expression in the young rabbits. LC-MS/MS identified 2286 proteins in the rabbit vocal folds of young and older adult rabbits combined. Of these, 177, 169, and 81 proteins were significantly (p ≤ 0.05) affected by age, hydration status, or the interaction of both factors, respectively. Analysis of the interaction effect revealed 32 proteins with opposite change patterns after dehydration between older and young rabbit vocal folds, while 31 proteins were differentially regulated only in the older adult rabbits and ten only in the young rabbits in response to systemic dehydration. The magnitude of changes for either up or downregulated proteins was higher in the older rabbits. These proteins are predominantly related to structural components of the extracellular matrix and muscle layer, suggesting a disturbance in the viscoelastic properties of aging vocal fold tissue, especially when subjected to systemic dehydration.

conclusionsWater restriction is a laboratory protocol to assess systemic dehydration-related changes in the vocal fold tissue that is translatable to human subjects. Our findings showed a higher number of proteins differentially regulated with a greater magnitude of change in the vocal folds of older adult rabbits in the presence of systemic dehydration compared to younger rabbits. The association of these proteins with vocal fold structure and biomechanical properties suggests that older human subjects may be more vulnerable to the effects of systemic dehydration on vocal function. The clinical implications of these protein changes warrant more investigation, but age should be taken into consideration when evaluating vocal treatment recommendations that interfere with body fluid balance.

Indexed as

DehydrationVocal CordsAgedAgingAnimalsChild, PreschoolChromatography, LiquidHumansInfantProteomicsRabbitsTandem Mass SpectrometryWaterWaterAgingDehydrationLarynxProteomeVocal foldsWater restrictionYoung

Identifiers

PMID36411412
PMCPMC9677652
OpenAlexW4309664738

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.