Evidence map›Paper›PMID 36411313›Full record

ArticleNature biotechnology2023

High-throughput retrieval of target sequences from complex clone libraries using CRISPRi.

Ján Burian, Vincent K Libis, Yozen A Hernandez, Liliana Guerrero-Porras, Melinda A Ternei, Sean F Brady

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature biotechnology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Ján BurianLaboratory of Genetically Encoded Small Molecules, Rockefeller University, New York, NY, USA.
Vincent K LibisLaboratory of Genetically Encoded Small Molecules, Rockefeller University, New York, NY, USA.ORCID http://orcid.org/0000-0001-8057-2196
Yozen A HernandezLaboratory of Genetically Encoded Small Molecules, Rockefeller University, New York, NY, USA.
Liliana Guerrero-PorrasLaboratory of Genetically Encoded Small Molecules, Rockefeller University, New York, NY, USA.
Melinda A TerneiLaboratory of Genetically Encoded Small Molecules, Rockefeller University, New York, NY, USA.ORCID http://orcid.org/0000-0002-5693-9944
Sean F BradyLaboratory of Genetically Encoded Small Molecules, Rockefeller University, New York, NY, USA. sbrady@rockefeller.edu.ORCID http://orcid.org/0000-0001-5967-8586

Funding

Discovery of Antibiotics from Soil Microbiomes Using MetagenomicsR35GM122559 · NIGMS · ROCKEFELLER UNIVERSITY · PI SEAN F BRADY · 2017 to 2026
$4.7M
NIGMS NIH HHS R35 GM122559
6 · The paper itself

Abstract

The capture of metagenomic DNA in large clone libraries provides the opportunity to study microbial diversity that is inaccessible using culture-dependent methods. In this study, we harnessed nuclease-deficient Cas9 to establish a CRISPR counter-selection interruption circuit (CCIC) that can be used to retrieve target clones from complex libraries. Combining modern sequencing methods with CCIC cloning allows for rapid physical access to the genetic diversity present in natural ecosystems.

Indexed as

EcosystemMetagenomicsClone Cells

Identifiers

PMID36411313
PMCPMC11042918

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.