Evidence map›Paper›PMID 36409589›Full record

ArticleThe Journal of infectious diseases2023

Molecular Characterization of BK Polyomavirus Replication in Allogeneic Hematopoietic Cell Transplantation Patients.

Karoline Leuzinger, Amandeep Kaur, Maud Wilhelm, Konstantin Frank, Caroline A Hillenbrand, Fabian H Weissbach, Hans H Hirsch

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of infectious diseases, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.6field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 17 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Karoline LeuzingerTransplantation and Clinical Virology, Department Biomedicine, University of Basel, Basel, Switzerland.
Amandeep KaurTransplantation and Clinical Virology, Department Biomedicine, University of Basel, Basel, Switzerland.
Maud WilhelmTransplantation and Clinical Virology, Department Biomedicine, University of Basel, Basel, Switzerland.
Konstantin FrankTransplantation and Clinical Virology, Department Biomedicine, University of Basel, Basel, Switzerland.
Caroline A HillenbrandTransplantation and Clinical Virology, Department Biomedicine, University of Basel, Basel, Switzerland.
Fabian H WeissbachTransplantation and Clinical Virology, Department Biomedicine, University of Basel, Basel, Switzerland.
Hans H HirschTransplantation and Clinical Virology, Department Biomedicine, University of Basel, Basel, Switzerland.ORCID 0000-0003-0883-0423
University of Basel · CH

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundHigh-level BK polyomavirus (BKPyV) replication in allogeneic hematopoietic cell transplantation (HCT) predicts failing immune control and BKPyV-associated hemorrhagic cystitis.

methodsTo identify molecular markers of BKPyV replication and disease, we scrutinized BKPyV DNA-loads in longitudinal urine and plasma pairs from 20 HCT patients using quantitative nucleic acid testing (QNAT), DNase-I treatment prior to QNAT, next-generation sequencing (NGS), and tested cell-mediated immunity.

resultsWe found that larger QNAT amplicons led to under-quantification and false-negatives results (P < .001). DNase-I reduced urine and plasma BKPyV-loads by >90% (P < .001), indicating non-encapsidated BKPyV genomes. DNase-resistant urine BKPyV-loads remained infectious in cell culture. BKPyV genome fragmentation of ≤250 bp impaired NGS coverage of genetic variation using 1000-bp and 5000-bp amplicons. Conversely, 250-bp amplicons captured viral minority variants. We identified genotype-specific and genotype-independent changes in capsid Vp1 or T-antigen predicted to escape from antibody neutralization or cytotoxic CD8 T-cells, respectively. Genotype-specific changes in immunodominant 9mers were associated with reduced or absent CD8 T-cell responses. Thus, failure to control BKPyV replication in HCT Patients may involve insufficient genotype-specific cytotoxic CD8 T-cell responses, potentially predictable by low neutralizing antibodies as well as genotype-independent immune escape.

conclusionsOur results provide new insights for patient evaluation and for designing immune protection through neutralizing antibodies, adoptive T-cell therapy, or vaccines.

Indexed as

BK VirusHematopoietic Stem Cell TransplantationPolyomavirus InfectionsAntibodies, NeutralizingCD8-Positive T-LymphocytesHumansAntibodies, NeutralizingBK polyomavirusBKPyVCD8epitopeHCThematopoietic cell transplantationhemorrhagic cystitisimmune escapeLTagneutralizing antibodyT cellVp1

Identifiers

PMID36409589
PMCPMC10093069
OpenAlexW4309509394

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.